Reactome | PTEN C124S [cytosol]

PTEN C124S [cytosol]

Stable Identifier

R-HSA-2317379

Type

Protein [EntityWithAccessionedSequence]

Species

Homo sapiens

Compartment

cytosol

Synonyms

PTEN Cys124Ser

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Disease (Homo sapiens)

- Diseases of signal transduction by growth factor receptors and second messengers (Homo sapiens)
  - PI3K/AKT Signaling in Cancer (Homo sapiens)
    - PTEN Loss of Function in Cancer (Homo sapiens)
      - PTEN cancer mutants do not dephosphorylate PIP3 (Homo sapiens)
        
        PTEN Mutants [cytosol] (Homo sapiens)
        
        PTEN Phosphatase Domain Missense Mutants [cytosol] (Homo sapiens)
        
        PTEN C124S [cytosol] (Homo sapiens)

General

PTEN missense mutation that results in the substitution of cysteine at position 124 with serine affects the conserved H-C-K/R-A-G-K-G-R sequence (corresponding to HCXXGXXR motif of protein tyrosine phosphatases) in the catalytic cleft of the PTEN phosphatase domain. The cystein residue in this motif, corresponding to C124 of human PTEN, 'attacks' the phosphate group of a substrate and forms a thio-phosphate intermediate during dephosphorylation reaction (Guan and Dixon 1991, Barford et al. 1994, Lee et al. 1999). PTEN C124S (Cys124Ser) mutant shows markedly decreased phosphoinositide phosphatase activity (Koul et al. 2002).

Literature References

PubMed ID	Title	Journal	Year
1654322	Evidence for protein-tyrosine-phosphatase catalysis proceeding via a cysteine-phosphate intermediate Guan, KL, Dixon, JE	J. Biol. Chem.	1991
8128219	Crystal structure of human protein tyrosine phosphatase 1B Barford, D, Flint, AJ, Tonks, NK	Science	1994
10555148	Crystal structure of the PTEN tumor suppressor: implications for its phosphoinositide phosphatase activity and membrane association Georgescu, MM, Pavletich, NP, Shi, Y, Maehama, T, Pandolfi, P, Yang, H, Lee, JO, Dixon, JE, Di Cristofano, A	Cell	1999
11948419	Motif analysis of the tumor suppressor gene MMAC/PTEN identifies tyrosines critical for tumor suppression and lipid phosphatase activity Yung, WK, Shi, Y, Jasser, SA, Davies, MA, Lu, Y, Koul, D, Mills, GB, Shen, R	Oncogene	2002

External Reference Information

External Reference

UniProt:P60484 PTEN

Gene Names

PTEN, MMAC1, TEP1

Chain

initiator methionine:, chain:2-403

Reference Genes

COSMIC (genes):PTEN PTEN

COSMIC (genes):TEP1 PTEN

dbSNP Gene:5728 PTEN

ENSEMBL:ENSG00000171862 PTEN

Participates

as a member of

PTEN Phosphatase Domain Missense Mutants [cytosol] (Homo sapiens)

Other forms of this molecule

K48polyUb-PTEN [cytosol]

PolyUb-PTEN [nucleoplasm]

PTEN H93R [cytosol]

PTEN C124R [cytosol]

PTEN H93Y [cytosol]

PTEN R130G [cytosol]

PTEN H93D [cytosol]

PTEN R130L [cytosol]

PTEN H93A [cytosol]

PTEN G129E [cytosol]

PTEN S170N [cytosol]

PTEN R173P [cytosol]

PTEN R130Q [cytosol]

PTEN H93Q [cytosol]

PTEN G129R [cytosol]

PTEN H123Y [cytosol]

PTEN S170R [cytosol]

PTEN R173C [cytosol]

PTEN R173H [cytosol]

PTEN C124Y [cytosol]

PTEN R130P [cytosol]

PTEN C124F [cytosol]

PTEN G129V [cytosol]

PTEN S170I [cytosol]

PTEN S170G [cytosol]

PTEN E99* [cytosol]

PTEN G165* [cytosol]

PTEN E157* [cytosol]

PTEN E150* [cytosol]

PTEN E43* [cytosol]

PTEN Q17* [cytosol]

PTEN E91* [cytosol]

PTEN S59* [cytosol]

PTEN E114* [cytosol]

PTEN L139* [cytosol]

PTEN Y176* [cytosol]

PTEN Y177* [cytosol]

PTEN Q149* [cytosol]

PTEN L146* [cytosol]

PTEN G20* [cytosol]

PTEN G127* [cytosol]

PTEN Y46* [cytosol]

PTEN L182* [cytosol]

PTEN Q97* [cytosol]

PTEN Q171* [cytosol]

PTEN G129* [cytosol]

PTEN E18* [cytosol]

PTEN Y180* [cytosol]

PTEN K163* [cytosol]

PTEN Y27* [cytosol]

PTEN Y65* [cytosol]

PTEN Y68* [cytosol]

PTEN K128* [cytosol]

PTEN Y138* [cytosol]

PTEN K125* [cytosol]

PTEN Y88* [cytosol]

PTEN Q110* [cytosol]

PTEN L23* [cytosol]

PTEN E7* [cytosol]

PTEN C105* [cytosol]

PTEN E73* [cytosol]

PTEN K62* [cytosol]

PTEN E40* [cytosol]

PTEN Y178* [cytosol]

PTEN R130* [cytosol]

PTEN W111* [cytosol]

PTEN R11* [cytosol]

PTEN K13* [cytosol]

PTEN Y174* [cytosol]

PTEN K147* [cytosol]

PTEN R84* [cytosol]

PTEN G293* [cytosol]

PTEN S287* [cytosol]

PTEN Q214* [cytosol]

PTEN S229* [cytosol]

PTEN Q219* [cytosol]

PTEN Q261* [cytosol]

PTEN E299* [cytosol]

PTEN Y336* [cytosol]

PTEN Q245* [cytosol]

PTEN Y225* [cytosol]

PTEN Y240* [cytosol]

PTEN C218* [cytosol]

PTEN C250* [cytosol]

PTEN L247* [cytosol]

PTEN G209* [cytosol]

PTEN C211* [cytosol]

PTEN G230* [cytosol]

PTEN Q298* [cytosol]

PTEN L320* [cytosol]

PTEN E242* [cytosol]

PTEN K332* [cytosol]

PTEN E284* [cytosol]

PTEN E291* [cytosol]

PTEN K330* [cytosol]

PTEN E314* [cytosol]

PTEN Y346* [cytosol]

PTEN C296* [cytosol]

PTEN W274* [cytosol]

PTEN R233* [cytosol]

PTEN R335* [cytosol]

PTEN [cytosol]

PTEN [nucleoplasm]

MonoUb-K13,K289-PTEN [nucleoplasm]

MonoUb-K13,K289-PTEN [cytosol]

K48polyUb-K289-PTEN [cytosol]

PolyUb-PTEN [cytosol]

K27polyUb-PTEN [cytosol]

p-3S,2T-PTEN [cytosol]

RibC-E40,E150,D326-PTEN [cytosol]

PolyUb-K324,K344,K349-RibC-E40,E150,D326-PTEN [cytosol]

p-Y336-PTEN [cytosol]

PTEN K344* [cytosol]

PTEN K221* [cytosol]

PTEN E288* [cytosol]

PTEN E235* [cytosol]

PTEN K263* [cytosol]

PTEN E307* [cytosol]

PTEN R189* [cytosol]

PTEN E256* [cytosol]

Modified Residues

Name

L-cysteine 124 replaced with L-serine

Coordinate

124

PsiMod

L-cysteine removal [MOD:01635]

A protein modification that effectively removes or replaces an L-cysteine.

L-serine residue [MOD:00025]

A protein modification that effectively converts a source amino acid residue to L-serine.

Disease

Name	Identifier	Synonyms
endometrial cancer	DOID:1380	primary malignant neoplasm of endometrium, neoplasm of endometrium (disorder), endometrial neoplasm, malignant endometrial neoplasm, endometrial Ca, malignant neoplasm of endometrium, tumor of Endometrium
cancer	DOID:162	malignant tumor, malignant neoplasm, primary cancer
thyroid cancer	DOID:1781	Thyroid gland neoplasm, thyroid cancer, thyroid neoplasm, neoplasm of thyroid gland, thyroid neoplasm, Thyroid neoplasm, malignant tumour of thyroid gland, malignant neoplasm of thyroid gland
glioblastoma multiforme	DOID:3068	GBM, adult glioblastoma multiforme, grade IV adult Astrocytic tumor, primary glioblastoma multiforme, spongioblastoma multiforme

Cross References

COSMIC

COSV64293195, COSV64288968

Interactors (26)

Accession	#Entities	Entities	Confidence Score	Evidence (IntAct)
UniProt:O14745 NHRF1			0.771	7
UniProt:P09619 PDGFRB	3	p-12Y-PDGFRB [plasma membrane] (R-HSA-186803) PDGFRB [plasma membrane] (R-HSA-186767) p-11Y-PDGFRB [plasma membrane] (R-HSA-8864039)	0.675	3
UniProt:Q15599 NHRF2			0.639	5
UniProt:P42685 FRK	4	p-Y387-FRK [cytosol] (R-HSA-8847965) FRK [specific granule lumen] (R-HSA-6801037) FRK [extracellular region] (R-HSA-6806235) FRK [azurophil granule lumen] (R-HSA-6799142)	0.639	7
UniProt:Q5TCQ9 MAGI3			0.633	6
UniProt:Q06830 PRDX1	3	PRDX1 [cytosol] (R-HSA-3341270) HOOS-C52-PRDX1 [cytosol] (R-HSA-5629198) p-T90-PRDX1 [cytosol] (R-HSA-8868564)	0.632	7
UniProt:P30260 CDC27	2	CDC27 [nucleoplasm] (R-HSA-174175) CDC27 [cytosol] (R-HSA-174073)	0.616	7
UniProt:P60484 PTEN	20	K48polyUb-PTEN [cytosol] (R-HSA-8874327) PolyUb-PTEN [nucleoplasm] (R-HSA-6782515) PTEN H93R [cytosol] (R-HSA-2318524) PTEN C124R [cytosol] (R-HSA-2317439) PTEN H93Y [cytosol] (R-HSA-2318520) PTEN R130G [cytosol] (R-HSA-2317339) PTEN H93D [cytosol] (R-HSA-2318550) PTEN R130L [cytosol] (R-HSA-2317430) PTEN H93A [cytosol] (R-HSA-2318544) PTEN G129E [cytosol] (R-HSA-2318509) PTEN S170N [cytosol] (R-HSA-2318408) PTEN R173P [cytosol] (R-HSA-2317480) PTEN R130Q [cytosol] (R-HSA-2317416) PTEN H93Q [cytosol] (R-HSA-2318565) PTEN G129R [cytosol] (R-HSA-2318501) PTEN H123Y [cytosol] (R-HSA-2318469) PTEN S170R [cytosol] (R-HSA-2318437) PTEN R173C [cytosol] (R-HSA-2317478) PTEN R173H [cytosol] (R-HSA-2317473) PTEN C124S [cytosol] (R-HSA-2317379)	0.615	9
UniProt:O43791 SPOP	1	SPOP [nucleoplasm] (R-HSA-5635035)	0.611	4
UniProt:P35226 BMI1	3	BMI1 [nucleoplasm] (R-HSA-389121) p-S-BMI1 [nucleoplasm] (R-HSA-3229116) monoSUMO1-K88-BMI1 [nucleoplasm] (R-HSA-4551729)	0.61	7
UniProt:Q9R1L5 MAST1			0.593	3
UniProt:O60307 MAST3			0.593	3
UniProt:Q60592 MAST2			0.593	4
UniProt:P46934 NEDD4	1	NEDD4 [cytosol] (R-HSA-975992)	0.591	4
UniProt:O88382 MAGI2			0.581	3
UniProt:P62136 PPP1CA	1	PPP1CA [cytosol] (R-HSA-163525)	0.524	2
UniProt:Q9JHL1 NHRF2			0.524	2
UniProt:Q9NRD5 PICK1	2	PICK1 [plasma membrane] (R-HSA-416989) PICK1 [endocytic vesicle membrane] (R-HSA-417017)	0.508	2
UniProt:Q9JK71 MAGI3			0.508	3
UniProt:Q9P0U4 CXXC1	1	CXXC1 [nucleoplasm] (R-HSA-535610)	0.508	2
UniProt:Q03135 CAV1	5	CAV1 [Golgi membrane] (R-HSA-204504) CAV1 [plasma membrane] (R-HSA-534994) CAV1 [lipid droplet] (R-HSA-163510) CAV1 [endocytic vesicle membrane] (R-HSA-535009) CAV1 [early endosome membrane] (R-HSA-5368451)	0.499	2
UniProt:Q9ULX6 AKAP8L	1	AKAP8L [nucleoplasm] (R-HSA-9821364)	0.499	2
UniProt:Q16643 DBN1	1	DBN1 [cytosol] (R-HSA-9714666)	0.471	5
UniProt:Q9UJX3 ANAPC7	2	ANAPC7 [nucleoplasm] (R-HSA-174204) ANAPC7 [cytosol] (R-HSA-174242)	0.462	2
UniProt:Q9UJX5 ANAPC4	2	ANAPC4 [cytosol] (R-HSA-174168) ANAPC4 [nucleoplasm] (R-HSA-174115)	0.462	2
UniProt:Q9UJX4 ANAPC5	2	ANAPC5 [cytosol] (R-HSA-174211) ANAPC5 [nucleoplasm] (R-HSA-174153)	0.462	2

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