Reactome | PIK3CA Q546P [cytosol]

PIK3CA Q546P [cytosol]

Stable Identifier

R-HSA-1500695

Type

Protein [EntityWithAccessionedSequence]

Species

Homo sapiens

Compartment

cytosol

Synonyms

PIK3CA Gln546Pro

Locations in the PathwayBrowser

Expand all

Disease (Homo sapiens)

- Diseases of signal transduction by growth factor receptors and second messengers (Homo sapiens)
  - PI3K/AKT Signaling in Cancer (Homo sapiens)
    - Constitutive Signaling by Aberrant PI3K in Cancer (Homo sapiens)
      - PI3K gain of function mutants phosphorylate PIP2 to PIP3 (Homo sapiens)
        
        PI3K mutants [plasma membrane] (Homo sapiens)
        
        PIK3R1:PIK3CA mutants [plasma membrane] (Homo sapiens)
        
        PIK3CA mutants [cytosol] (Homo sapiens)
        
        PIK3CA helical domain mutants [cytosol] (Homo sapiens)
        
        PIK3CA Q546P [cytosol] (Homo sapiens)
      - PI3K inhibitors block PI3K catalytic activity (Homo sapiens)
        
        PI3K Inhibitors:PI3K [cytosol] (Homo sapiens)
        
        PI3K mutants,Activator:PI3K [plasma membrane] (Homo sapiens)
        
        PI3K mutants [plasma membrane] (Homo sapiens)
        
        PIK3R1:PIK3CA mutants [plasma membrane] (Homo sapiens)
        
        PIK3CA mutants [cytosol] (Homo sapiens)
        
        PIK3CA helical domain mutants [cytosol] (Homo sapiens)
        
        PIK3CA Q546P [cytosol] (Homo sapiens)
        
        PI3K mutants,Activator:PI3K [plasma membrane] (Homo sapiens)
        
        PI3K mutants [plasma membrane] (Homo sapiens)
        
        PIK3R1:PIK3CA mutants [plasma membrane] (Homo sapiens)
        
        PIK3CA mutants [cytosol] (Homo sapiens)
        
        PIK3CA helical domain mutants [cytosol] (Homo sapiens)
        
        PIK3CA Q546P [cytosol] (Homo sapiens)

General

Based on structural and functional studies of PIK3CA Q546K mutant (Miled et al. 2007), substitution of glutamine residue at position 546 of PIK3CA with proline is expected to disrupt inhibitory interaction between the helical domain of PIK3CA and the nSH2 domain of PIK3R1, resulting in constitutive activity of the PI3K complex containing PIK3CA Q546P mutant.

Literature References

PubMed ID	Title	Journal	Year
17626883	Mechanism of two classes of cancer mutations in the phosphoinositide 3-kinase catalytic subunit Inbar, Y, Miled, N, Hon, WC, Williams, RL, Yan, Y, Backer, JM, Zvelebil, M, Wolfson, HJ, Schneidman-Duhovny, D, Perisic, O	Science	2007
15016963	High frequency of mutations of the PIK3CA gene in human cancers Ptak, J, Samuels, Y, Silliman, N, Yan, H, Gazdar, A, Riggins, GJ, Markowitz, S, Powell, SM, Szabo, S, Wang, Z, Willson, JKV, Bardelli, A, Kinzler, KW, Vogelstein, B, Velculescu, VE	Science	2004

External Reference Information

External Reference

UniProt:P42336 PIK3CA

Gene Names

PIK3CA

Chain

chain:1-1068

Reference Genes

BioGPS Gene:5290 PIK3CA

COSMIC (genes):PIK3CA PIK3CA

CTD Gene:5290 PIK3CA

dbSNP Gene:5290 PIK3CA

ENSEMBL:ENSG00000121879 PIK3CA

HGNC:8975 PIK3CA

KEGG Gene (Homo sapiens):5290 PIK3CA

Monarch:5290 PIK3CA

NCBI Gene:5290 PIK3CA

OMIM:171834 PIK3CA

UCSC:P42336 PIK3CA

Reference Transcript

Other Identifiers

0002900288

11745214_a_at

11763283_at

16948209

17122920

17122922

204369_at

231854_at

235980_at

2653841

2653852

2653853

2653855

2653856

2653857

2653858

2653860

2653861

2653862

2653864

2653865

2653866

2653867

2653868

2653869

2653871

2653872

2653873

2653875

2653876

2653877

2653879

2653880

2653881

2653882

2653883

2653884

2653885

2653890

3935885

3935887

3935891

3935893

3935895

3935897

40704_at

41969_at

5290

55954_at

8084016

81516_at

81657_at

83177_at

90039_at

A_14_P113684

A_14_P127878

A_14_P128410

A_21_P0012238

A_23_P92057

GE60386

GE88555

GO:0000165

GO:0000166

GO:0000902

GO:0001525

GO:0001889

GO:0001932

GO:0001944

GO:0002376

GO:0003013

GO:0003674

GO:0004674

GO:0005515

GO:0005524

GO:0005575

GO:0005622

GO:0005737

GO:0005829

GO:0005886

GO:0005942

GO:0005943

GO:0005944

GO:0005975

GO:0006006

GO:0006091

GO:0006259

GO:0006464

GO:0006468

GO:0006629

GO:0006661

GO:0006810

GO:0006909

GO:0006914

GO:0006950

GO:0007010

GO:0007155

GO:0007165

GO:0007173

GO:0007186

GO:0007411

GO:0008150

GO:0008219

GO:0009056

GO:0009058

GO:0010468

GO:0010592

GO:0014065

GO:0014068

GO:0014704

GO:0016020

GO:0016192

GO:0016242

GO:0016301

GO:0016303

GO:0016310

GO:0016477

GO:0016740

GO:0019221

GO:0022607

GO:0030027

GO:0030036

GO:0030154

GO:0030168

GO:0030234

GO:0030295

GO:0030835

GO:0031295

GO:0032008

GO:0032147

GO:0032991

GO:0033138

GO:0034641

GO:0035004

GO:0035005

GO:0035994

GO:0036092

GO:0038028

GO:0038084

GO:0038095

GO:0038096

GO:0038128

GO:0040007

GO:0040011

GO:0040014

GO:0042592

GO:0043167

GO:0043276

GO:0043457

GO:0043491

GO:0043524

GO:0043542

GO:0043560

GO:0044029

GO:0044281

GO:0046854

GO:0046934

GO:0048010

GO:0048015

GO:0048471

GO:0048646

GO:0048856

GO:0048870

GO:0050852

GO:0050900

GO:0051897

GO:0052742

GO:0052812

GO:0055119

GO:0060048

GO:0060612

GO:0071333

GO:0071464

GO:0086003

GO:0097009

GO:0106310

GO:0106311

GO:0110053

GO:2000270

GO:2000653

GO:2000811

HMNXSV003040760

Hs.120905.0.S1_3p_at

Hs.122419.0.S1_3p_at

ILMN_1705396

ILMN_1705468

PH_hs_0031379

TC03000951.hg

TC03002674.hg

TC22000929.hg

Z29090_at

g5453891_3p_at

Participates

as a candidate of

PIK3CA helical domain mutants [cytosol] (Homo sapiens)

Other forms of this molecule

PIK3CA H1047L [cytosol]

PIK3CA H1047R [cytosol]

PIK3CA M1043I [cytosol]

PIK3CA M1043T [cytosol]

PIK3CA M1043V [cytosol]

PIK3CA H1047Y [cytosol]

PIK3CA E542K [cytosol]

PIK3CA Q546K [cytosol]

PIK3CA E545A [cytosol]

PIK3CA E545K [cytosol]

PIK3CA E545Q [cytosol]

PIK3CA Q546E [cytosol]

PIK3CA E545V [cytosol]

PIK3CA E542V [cytosol]

PIK3CA Q546L [cytosol]

PIK3CA E545G [cytosol]

PIK3CA Q546H [cytosol]

PIK3CA Q546R [cytosol]

PIK3CA E542Q [cytosol]

PIK3CA R38H [cytosol]

PIK3CA R38S [cytosol]

PIK3CA R38G [cytosol]

PIK3CA R38C [cytosol]

PIK3CA [cytosol]

Modified Residues

Name

L-glutamine 546 replaced with L-proline

Coordinate

546

PsiMod

L-glutamine removal [MOD:01637]

A protein modification that effectively removes or replaces an L-glutamine.

L-proline residue [MOD:00024]

A protein modification that effectively converts a source amino acid residue to L-proline.

Disease

Name	Identifier	Synonyms
endometrial cancer	DOID:1380	primary malignant neoplasm of endometrium, neoplasm of endometrium (disorder), endometrial neoplasm, malignant endometrial neoplasm, endometrial Ca, malignant neoplasm of endometrium, tumor of Endometrium
ovarian cancer	DOID:2394	ovarian neoplasm, malignant tumour of ovary, neoplasm of ovary (disorder), ovary neoplasm, primary malignant neoplasm of ovary and other uterine adnexa (disorder), ovarian cancer, tumor of the Ovary, ovary cancer, malignant Ovarian tumor, primary ovarian cancer, malignant tumor of ovary (disorder)
cancer	DOID:162	malignant tumor, malignant neoplasm, primary cancer
breast cancer	DOID:1612	malignant tumor of the breast, mammary cancer, malignant neoplasm of breast, mammary tumor, primary breast cancer, breast cancer, Ca breast - NOS, breast tumor
brain glioma	DOID:0060108
large intestine cancer	DOID:5672	malignant Colorectal tumor, malignant neoplasm of large intestine, NOS, malignant tumor of large intestine (disorder), malignant neoplasm of colon with rectum, malignant neoplasm of large intestine NOS

Cross References

RefSeq

NP_006209.2, XP_011511196.1, XP_006713721.1

ZINC - World Drugs

PK3CA_HUMAN

Guide to Pharmacology - Targets

2153

OpenTargets

ENSG00000121879

ZINC - FDA approved

PK3CA_HUMAN

ZINC - Substances

PK3CA_HUMAN

ZINC target

P42336

ZINC - Biogenic

PK3CA_HUMAN

PRO

P42336

PDB

6OAC, 5SXF, 5SXD, 5DXH, 5SXE, 2RD0, 5UL1, 4L1B, 2ENQ, 4TUU, 4TV3, 5DXT, 7K6N, 7K6O, 5FI4, 3HIZ, 4WAF, 4L23, 7K6M, 5UBR, 5SW8, 5UK8, 3ZIM, 4ZOP, 7K71, 7JIU, 6GVH, 6GVI, 5UKJ, 5SXI, 6GVF, 6GVG, 5SWG, 6PYS, 5SWP, 5XGH, 5SWO, 5SWR, 5XGI, 4L2Y, 5SXJ, 6NCT, 5ITD, 5SXK, 5SX9, 4JPS, 5XGJ, 5SWT, 4YKN, 5SX8, 5SXB, 4OVV, 6VO7, 5SXC, 4OVU, 3HHM, 5SXA

ZINC - Investigational

PK3CA_HUMAN

ZINC - Metabolites

PK3CA_HUMAN

COSMIC

COSV55875400

IntEnz

2.7.1.137

HPA

ENSG00000121879-PIK3CA

GeneCards

P42336

Ensembl

ENSG00000121879, ENSP00000263967, ENST00000263967

Pharos - Targets

P42336

Orphanet

15093

ZINC - Predictions - Purchasable

PK3CA_HUMAN

HMDB Protein

HMDBP01236

Interactors (13)

Accession	#Entities	Entities	Confidence Score	Evidence (IntAct)
UniProt:P27986 PIK3R1	15	PIK3R1 G376R [plasma membrane] (R-HSA-2399717) PIK3R1 K379E [plasma membrane] (R-HSA-8944148) PIK3R1 Y463_L466del [plasma membrane] (R-HSA-2399546) PIK3R1 H450_E451del [plasma membrane] (R-HSA-2399545) PIK3R1 R574_T576del [plasma membrane] (R-HSA-2399544) PIK3R1 D560Y [plasma membrane] (R-HSA-2399421) PIK3R1 N564D [plasma membrane] (R-HSA-2399427) PIK3R1 K459del [plasma membrane] (R-HSA-2399554) PIK3R1 N564K [plasma membrane] (R-HSA-2399431) PIK3R1 D560H [plasma membrane] (R-HSA-2399432) PIK3R1 R574I [plasma membrane] (R-HSA-2399536) PIK3R1 R574T [plasma membrane] (R-HSA-2399540) K63polyUb-PIK3R1 [cytosol] (R-HSA-3080572) PIK3R1 [cytosol] (R-HSA-74789) PIK3R1 [plasma membrane] (R-HSA-198365)	0.961	23
UniProt:P35568 IRS1	5	p-Y-IRS1 [plasma membrane] (R-HSA-198366) p-Y-IRS1 [cytosol] (R-HSA-74772) IRS1 [cytosol] (R-HSA-74769) IRS1 [plasma membrane] (R-HSA-2425392) p-6Y-IRS1 [plasma membrane] (R-HSA-2445090)	0.755	21
UniProt:P62993 GRB2	1	GRB2 [cytosol] (R-HSA-8983328)	0.742	5
UniProt:Q92569 PIK3R3	2	PIK3R3 [cytosol] (R-HSA-205223) K63polyUb-PIK3R3 [cytosol] (R-HSA-3080574)	0.738	5
UniProt:O00459 PIK3R2	2	K63polyUb-PIK3R2 [cytosol] (R-HSA-3080554) PIK3R2 [cytosol] (R-HSA-74791)	0.725	9
UniProt:P21860 ERBB3			0.564	3
UniProt:P27986-2 PIK3R1			0.556	3
UniProt:P01100 FOS	2	FOS [nucleoplasm] (R-HSA-450216) p-T325,T331,S362,S374-FOS [nucleoplasm] (R-HSA-450283)	0.556	3
UniProt:P54253 ATX1			0.556	3
UniProt:P42858 HTT	1	HTT [nucleoplasm] (R-HSA-9023590)	0.556	3
UniProt:P54252 ATXN3	2	ATXN3 [nucleoplasm] (R-HSA-6783285) ATXN3 [cytosol] (R-HSA-5625607)	0.556	3
UniProt:P03496 NS	2	NS1 [nucleoplasm] (R-FLU-169113) NS1 [cytosol] (R-FLU-169118)	0.527	7
UniProt:P42336 PIK3CA	20	PIK3CA H1047L [cytosol] (R-HSA-1500714) PIK3CA H1047R [cytosol] (R-HSA-1500716) PIK3CA M1043I [cytosol] (R-HSA-1500719) PIK3CA M1043T [cytosol] (R-HSA-2395934) PIK3CA M1043V [cytosol] (R-HSA-2395932) PIK3CA H1047Y [cytosol] (R-HSA-1500720) PIK3CA E542K [cytosol] (R-HSA-1500701) PIK3CA Q546K [cytosol] (R-HSA-1500705) PIK3CA E545A [cytosol] (R-HSA-2362355) PIK3CA E545K [cytosol] (R-HSA-1500706) PIK3CA E545Q [cytosol] (R-HSA-2362362) PIK3CA Q546E [cytosol] (R-HSA-2362383) PIK3CA E545V [cytosol] (R-HSA-2362365) PIK3CA E542V [cytosol] (R-HSA-2328074) PIK3CA Q546L [cytosol] (R-HSA-2362375) PIK3CA E545G [cytosol] (R-HSA-1500709) PIK3CA Q546H [cytosol] (R-HSA-2362374) PIK3CA Q546R [cytosol] (R-HSA-2362373) PIK3CA Q546P [cytosol] (R-HSA-1500695) PIK3CA E542Q [cytosol] (R-HSA-2328003)	0.462	3

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