Maturation of replicase proteins

Stable Identifier
R-HSA-9694301
Type
Pathway
Species
Homo sapiens
Related Species
Severe acute respiratory syndrome coronavirus 2
ReviewStatus
5/5
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Maturation of replicase proteins
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This COVID-19 pathway has been created by a combination of computational inference from SARS-CoV-1 data (https://reactome.org/documentation/inferred-events) and manual curation, as described in the summation for the overall SARS-CoV-2 infection pathway.

Production of polyprotein fragments (so called replicase proteins) involves the repeated autocleavage of the polyprotein, liberating the two endopeptidases that finally cleave all fragments efficiently. Only nsp3 and nsp4 are post-translationally modified, they are glycosylated (Muramatsu et al, 2015; Harcourt et al, 2004; Oostra et al, 2007).
Literature References
PubMed ID Title Journal Year
17855519 Localization and membrane topology of coronavirus nonstructural protein 4: involvement of the early secretory pathway in replication

Oostra, M, te Lintelo, EG, Deijs, M, Verheije, MH, Rottier, PJ, de Haan, CA

J. Virol. 2007
27799534 SARS-CoV 3CL protease cleaves its C-terminal autoprocessing site by novel subsite cooperativity

Muramatsu, T, Takemoto, C, Kim, YT, Wang, H, Nishii, W, Terada, T, Shirouzu, M, Yokoyama, S

Proc. Natl. Acad. Sci. U.S.A. 2016
15564471 Identification of severe acute respiratory syndrome coronavirus replicase products and characterization of papain-like protease activity

Harcourt, BH, Jukneliene, D, Kanjanahaluethai, A, Bechill, J, Severson, KM, Smith, CM, Rota, PA, Baker, SC

J. Virol. 2004
Participants
Events
Participates
as an event of
Inferred From
Maturation of replicase proteins (Homo sapiens)
Disease
Name Identifier Synonyms
COVID-19 DOID:0080600 2019 Novel Coronavirus (2019-nCoV), Wuhan seafood market pneumonia virus infection, 2019-nCoV infection, Wuhan coronavirus infection
Cross References
Mondo
Authored
Stephan, R  (2020-08-28)
Reviewed
Acencio, ML  (2020-09-09)
Created
Cook, J  (2020-07-07)
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