Reactome | Recognition and association of DNA glycosylase with site containing an affected purine

Recognition and association of DNA glycosylase with site containing an affected purine

Stable Identifier

R-HSA-110330

Type

Pathway

Species

Homo sapiens

Compartment

nucleoplasm

ReviewStatus

5/5

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Recognition and association of DNA glycosylase with site containing an affected purine

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The recognition and removal of an altered base by a DNA glycosylase is thought to involve the diffusion of the enzyme along the minor grove of the DNA molecule. The enzyme presumably compresses the backbone of the affected DNA strand at the site of damage. This compression is thought to result in an outward rotation of the damaged residue into a "pocket" of the enzyme that recognizes and cleaves the altered base from the backbone (Slupphaug et al. 1996, Parikh et al. 1998).

Literature References

PubMed ID	Title	Journal	Year
8900285	A nucleotide-flipping mechanism from the structure of human uracil-DNA glycosylase bound to DNA Kavli, B, Tainer, JA, Mol, CD, Krokan, HE, Arvai, AS, Slupphaug, G	Nature	1996
9724657	Base excision repair initiation revealed by crystal structures and binding kinetics of human uracil-DNA glycosylase with DNA Tainer, JA, Bharati, S, Krokan, HE, Mol, CD, Slupphaug, G, Parikh, SS	EMBO J	1998