Reactome | mRNA Splicing

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mRNA Splicing - Major Pathway Show undefined attributes

authored	[InstanceEdit:72076] Krainer, AR, 2003-06-05 08:30:23 [InstanceEdit:75078] Joshi-Tope, G, 2003-08-22 11:27:00 [InstanceEdit:9769907] May, Bruce, 2022-03-22
compartment	[Compartment:7660] nucleoplasm
created	[InstanceEdit:72076] Krainer, AR, 2003-06-05 08:30:23
dbId	72163
displayName	mRNA Splicing - Major Pathway
doi	10.3180/R-HSA-72163.3
edited	[InstanceEdit:9769907] May, Bruce, 2022-03-22
eventOf	[Pathway:R-HSA-72172] mRNA Splicing - Homo sapiens
followingEvent	[Pathway:R-HSA-72187] mRNA 3'-end processing - Homo sapiens
goBiologicalProcess	[GO_BiologicalProcess:77621] mRNA splicing, via spliceosome
hasDiagram	false
hasEHLD	false
hasEvent	[BlackBoxEvent:R-HSA-9770119] Formation of the Spliceosomal E complex - Homo sapiens [BlackBoxEvent:R-HSA-9770129] Formation of the Spliceosomal A complex - Homo sapiens [BlackBoxEvent:R-HSA-9770132] Formation of the Spliceosomal Pre-B complex - Homo sapiens [BlackBoxEvent:R-HSA-9770142] Formation of the Spliceosomal B complex - Homo sapiens [BlackBoxEvent:R-HSA-9770145] Formation of the Spliceosomal Bact complex - Homo sapiens [BlackBoxEvent:R-HSA-9770131] Formation of the Spliceosomal B* complex - Homo sapiens [BlackBoxEvent:R-HSA-9794542] Formation of the Spliceosomal C complex containing intron lariat - Homo sapiens [BlackBoxEvent:R-HSA-9770141] Formation of the Spliceosomal C* complex - Homo sapiens [BlackBoxEvent:R-HSA-9770236] Formation of the Spliceosomal P complex and exon ligation - Homo sapiens [BlackBoxEvent:R-HSA-9770847] Spliceosomal P complex dissociates yielding the intron-containing complex (ILS) and the spliced mRNP (new) - Homo sapiens [Reaction:R-HSA-9772351] Disassembly of the Intron Lariat Spliceosome (new) - Homo sapiens
isInDisease	false
isInferred	false
lastUpdatedDate	2023-03-29
literatureReference	[LiteratureReference:9709004] Cryo-EM snapshots of the human spliceosome reveal structural adaptions for splicing regulation [LiteratureReference:9773071] How Is Precursor Messenger RNA Spliced by the Spliceosome? [LiteratureReference:9773068] RNA Splicing by the Spliceosome [LiteratureReference:9773072] A Day in the Life of the Exon Junction Complex [LiteratureReference:9773074] Molecular Mechanisms of pre-mRNA Splicing through Structural Biology of the Spliceosome [LiteratureReference:9773073] Spliceosome structure and function [LiteratureReference:9773231] Intron-exon structures of eukaryotic model organisms [LiteratureReference:9771902] A compensatory base change in U1 snRNA suppresses a 5' splice site mutation [LiteratureReference:9770231] Association of U2 snRNP with the spliceosomal complex E [LiteratureReference:9770219] Functional association of U2 snRNP with the ATP-independent spliceosomal complex E [LiteratureReference:72109] Protein composition of human prespliceosomes isolated by a tobramycin affinity-selection method. [LiteratureReference:72117] Large-scale proteomic analysis of the human spliceosome. [LiteratureReference:8865895] Dynamic protein-protein interaction wiring of the human spliceosome [LiteratureReference:8850757] Functional mammalian spliceosomal complex E contains SMN complex proteins in addition to U1 and U2 snRNPs [LiteratureReference:8865896] Mammalian splicing factor SF1 interacts with SURP domains of U2 snRNP-associated proteins [LiteratureReference:9771897] Crystal structure of human U1 snRNP, a small nuclear ribonucleoprotein particle, reveals the mechanism of 5' splice site recognition [LiteratureReference:9771907] Noncanonical registers and base pairs in human 5' splice-site selection [LiteratureReference:9771890] Mammalian pre-mRNA branch site selection by U2 snRNP involves base pairing [LiteratureReference:9771893] U2AF65 recruits a novel human DEAD box protein required for the U2 snRNP-branchpoint interaction [LiteratureReference:5420912] Mass spectrometry and EST-database searching allows characterization of the multi-protein spliceosome complex [LiteratureReference:9770232] Prp5 bridges U1 and U2 snRNPs and enables stable U2 snRNP association with intron RNA [LiteratureReference:5566497] Composition and three-dimensional EM structure of double affinity-purified, human prespliceosomal A complexes [LiteratureReference:9771903] Distinct activities of the DExD/H-box splicing factor hUAP56 facilitate stepwise assembly of the spliceosome [LiteratureReference:9770261] Stoichiometries of U2AF35, U2AF65 and U2 snRNP reveal new early spliceosome assembly pathways [LiteratureReference:9771887] Molecular architecture of the human 17S U2 snRNP [LiteratureReference:9771906] Evidence for base-pairing between mammalian U2 and U6 small nuclear ribonucleoprotein particles [LiteratureReference:927736] A simple whole cell lysate system for in vitro splicing reveals a stepwise assembly of the exon-exon junction complex [LiteratureReference:8850665] Aly and THO are required for assembly of the human TREX complex and association of TREX components with the spliced mRNA [LiteratureReference:9772622] Structural and functional analysis of the human spliceosomal DEAD-box helicase Prp28 [LiteratureReference:9770241] A spliceosome intermediate with loosely associated tri-snRNP accumulates in the absence of Prp28 ATPase activity [LiteratureReference:9769529] Structures of the human pre-catalytic spliceosome and its precursor spliceosome [LiteratureReference:9769531] Mechanism of 5' splice site transfer for human spliceosome activation [LiteratureReference:9769583] Structural Insights into Nuclear pre-mRNA Splicing in Higher Eukaryotes [LiteratureReference:9769520] Mechanism of protein-guided folding of the active site U2/U6 RNA during spliceosome activation [LiteratureReference:9772614] The 100-kda U5 snRNP protein (hPrp28p) contacts the 5' splice site through its ATPase site [LiteratureReference:5566502] Protein composition and electron microscopy structure of affinity-purified human spliceosomal B complexes isolated under physiological conditions [LiteratureReference:8865916] Isolation of an active step I spliceosome and composition of its RNP core [LiteratureReference:9772724] Exon, intron and splice site locations in the spliceosomal B complex [LiteratureReference:6806809] Characterization of purified human Bact spliceosomal complexes reveals compositional and morphological changes during spliceosome activation and first step catalysis [LiteratureReference:9770574] Mass spectrometry-based relative quantification of proteins in precatalytic and catalytically active spliceosomes by metabolic labeling (SILAC), chemical labeling (iTRAQ), and label-free spectral count [LiteratureReference:9772624] Cryo-EM Structure of a Pre-catalytic Human Spliceosome Primed for Activation [LiteratureReference:9769580] Structure of the human activated spliceosome in three conformational states [LiteratureReference:6806782] Spliceosome assembly involves the binding and release of U4 small nuclear ribonucleoprotein [LiteratureReference:9772623] The human U5-200kD DEXH-box protein unwinds U4/U6 RNA duplices in vitro [LiteratureReference:5420900] Functional analysis of the human CDC5L complex and identification of its components by mass spectrometry [LiteratureReference:9770594] Semiquantitative proteomic analysis of the human spliceosome via a novel two-dimensional gel electrophoresis method [LiteratureReference:9769577] Structure and Conformational Dynamics of the Human Spliceosomal B^act Complex [LiteratureReference:9770585] Structural and functional insights into CWC27/CWC22 heterodimer linking the exon junction complex to spliceosomes [LiteratureReference:72132] Purification and characterization of native spliceosomes suitable for three-dimensional structural analysis. [LiteratureReference:5566504] Small nuclear ribonucleoprotein remodeling during catalytic activation of the spliceosome [LiteratureReference:157383] 5' exon interactions within the human spliceosome establish a framework for exon junction complex structure and assembly [LiteratureReference:8850870] Contribution of DEAH-box protein DHX16 in human pre-mRNA splicing [LiteratureReference:9772093] Human spliceosomal protein CWC22 plays a role in coupling splicing to exon junction complex deposition and nonsense-mediated decay [LiteratureReference:9772088] Human CWC22 escorts the helicase eIF4AIII to spliceosomes and promotes exon junction complex assembly [LiteratureReference:8865939] CWC22 connects pre-mRNA splicing and exon junction complex assembly [LiteratureReference:9772613] Structure of a human catalytic step I spliceosome [LiteratureReference:9772742] The mammalian homologue of Prp16p is overexpressed in a cell line tolerant to Leflunomide, a new immunoregulatory drug effective against rheumatoid arthritis [LiteratureReference:9772746] Human homologs of yeast prp16 and prp17 reveal conservation of the mechanism for catalytic step II of pre-mRNA splicing [LiteratureReference:6806800] Rearrangements within human spliceosomes captured after exon ligation [LiteratureReference:9770783] An Atomic Structure of the Human Spliceosome [LiteratureReference:8984694] The protein Aly links pre-messenger-RNA splicing to nuclear export in metazoans [LiteratureReference:9769585] A human postcatalytic spliceosome structure reveals essential roles of metazoan factors for exon ligation [LiteratureReference:9769600] Structures of the human spliceosomes before and after release of the ligated exon [LiteratureReference:9772736] A human RNA helicase-like protein, HRH1, facilitates nuclear export of spliced mRNA by releasing the RNA from the spliceosome [LiteratureReference:9770592] Protein composition of human mRNPs spliced in vitro and differential requirements for mRNP protein recruitment [LiteratureReference:5566492] Isolation and characterization of post-splicing lariat-intron complexes [LiteratureReference:9772987] Human cactin interacts with DHX8 and SRRM2 to assure efficient pre-mRNA splicing and sister chromatid cohesion [LiteratureReference:9771082] Structural and functional characterisation of human RNA helicase DHX8 provides insights into the mechanism of RNA-stimulated ADP release [LiteratureReference:9772984] TFIP11 interacts with mDEAH9, an RNA helicase involved in spliceosome disassembly [LiteratureReference:9772981] Identification of a novel component C2ORF3 in the lariat-intron complex: lack of C2ORF3 interferes with pre-mRNA splicing via intron turnover pathway [LiteratureReference:9772985] Structural basis for DEAH-helicase activation by G-patch proteins
maxDepth	2
modified	[InstanceEdit:11108703] Weiser, Joel, 2026-03-16
name	mRNA Splicing - Major Pathway pre-mRNA splicing U2 Dependent Splicing
orthologousEvent	[Pathway:R-MMU-72163] mRNA Splicing - Major Pathway - Mus musculus [Pathway:R-PFA-72163] mRNA Splicing - Major Pathway - Plasmodium falciparum [Pathway:R-SPO-72163] mRNA Splicing - Major Pathway - Schizosaccharomyces pombe [Pathway:R-DDI-72163] mRNA Splicing - Major Pathway - Dictyostelium discoideum [Pathway:R-CEL-72163] mRNA Splicing - Major Pathway - Caenorhabditis elegans [Pathway:R-DME-72163] mRNA Splicing - Major Pathway - Drosophila melanogaster [Pathway:R-GGA-72163] mRNA Splicing - Major Pathway - Gallus gallus [Pathway:R-XTR-72163] mRNA Splicing - Major Pathway - Xenopus tropicalis [Pathway:R-DRE-72163] mRNA Splicing - Major Pathway - Danio rerio [Pathway:R-SSC-72163] mRNA Splicing - Major Pathway - Sus scrofa [Pathway:R-BTA-72163] mRNA Splicing - Major Pathway - Bos taurus [Pathway:R-CFA-72163] mRNA Splicing - Major Pathway - Canis familiaris [Pathway:R-RNO-72163] mRNA Splicing - Major Pathway - Rattus norvegicus
releaseDate	2004-07-06
reviewStatus	[ReviewStatus:9821382] five stars
reviewed	[InstanceEdit:9818818] Charenton, Cl�ment, 2022-10-20
revised	[InstanceEdit:9769907] May, Bruce, 2022-03-22
schemaClass	Pathway
species	[Species:48887] Homo sapiens
stId	R-HSA-72163
summation	[Summation:9773064] Eukaryotic genes are transcribed to yield pre-mRNAs that are...
updateTrackers	[UpdateTracker:9775673] Update Tracker - [Pathway:72163] mRNA Splicing - Major Pathway - v78:[updateContainedRLE] [UpdateTracker:9784010] Update Tracker - [Pathway:72163] mRNA Splicing - Major Pathway - v55:[updateContainedRLE] [UpdateTracker:9783517] Update Tracker - [Pathway:72163] mRNA Splicing - Major Pathway - v56:[updateContainedRLE] [UpdateTracker:9786512] Update Tracker - [Pathway:72163] mRNA Splicing - Major Pathway - v49:[updateContainedRLE] [UpdateTracker:9783301] Update Tracker - [Pathway:72163] mRNA Splicing - Major Pathway - v57:[updateContainedRLE] [UpdateTracker:9774821] Update Tracker - [Pathway:72163] mRNA Splicing - Major Pathway - v81:[updateContainedRLE] [UpdateTracker:9846898] Update Tracker - [Pathway:72163] mRNA Splicing - Major Pathway - v83:[addLiteratureReference, add_removeHasEvent, add_removeSummation] [UpdateTracker:9781554] Update Tracker - [Pathway:72163] mRNA Splicing - Major Pathway - v60:[updateContainedRLE] [UpdateTracker:9790388] Update Tracker - [Pathway:72163] mRNA Splicing - Major Pathway - v33:[addHasEvent] [UpdateTracker:9846781] Update Tracker - [Pathway:72163] mRNA Splicing - Major Pathway - v84:[modifyText] [UpdateTracker:9775172] Update Tracker - [Pathway:72163] mRNA Splicing - Major Pathway - v80:[updateContainedRLE] [UpdateTracker:9928477] Update Tracker - [Pathway:72163] mRNA Splicing - Major Pathway - v91:[removeHasEvent] [UpdateTracker:9784619] Update Tracker - [Pathway:72163] mRNA Splicing - Major Pathway - v53:[updateContainedRLE]

Referrals

(precedingEvent)	[Pathway:R-HSA-72187] mRNA 3'-end processing
(hasEvent)	[Pathway:R-HSA-72172] mRNA Splicing
(updatedInstance)	[UpdateTracker:9775673] Update Tracker - [Pathway:72163] mRNA Splicing - Major Pathway - v78:[updateContainedRLE] [UpdateTracker:9784010] Update Tracker - [Pathway:72163] mRNA Splicing - Major Pathway - v55:[updateContainedRLE] [UpdateTracker:9783517] Update Tracker - [Pathway:72163] mRNA Splicing - Major Pathway - v56:[updateContainedRLE] [UpdateTracker:9786512] Update Tracker - [Pathway:72163] mRNA Splicing - Major Pathway - v49:[updateContainedRLE] [UpdateTracker:9783301] Update Tracker - [Pathway:72163] mRNA Splicing - Major Pathway - v57:[updateContainedRLE] [UpdateTracker:9774821] Update Tracker - [Pathway:72163] mRNA Splicing - Major Pathway - v81:[updateContainedRLE] [UpdateTracker:9846898] Update Tracker - [Pathway:72163] mRNA Splicing - Major Pathway - v83:[addLiteratureReference, add_removeHasEvent, add_removeSummation] [UpdateTracker:9781554] Update Tracker - [Pathway:72163] mRNA Splicing - Major Pathway - v60:[updateContainedRLE] [UpdateTracker:9790388] Update Tracker - [Pathway:72163] mRNA Splicing - Major Pathway - v33:[addHasEvent] [UpdateTracker:9846781] Update Tracker - [Pathway:72163] mRNA Splicing - Major Pathway - v84:[modifyText] [UpdateTracker:9775172] Update Tracker - [Pathway:72163] mRNA Splicing - Major Pathway - v80:[updateContainedRLE] [UpdateTracker:9928477] Update Tracker - [Pathway:72163] mRNA Splicing - Major Pathway - v91:[removeHasEvent] [UpdateTracker:9784619] Update Tracker - [Pathway:72163] mRNA Splicing - Major Pathway - v53:[updateContainedRLE]

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