Reactome | Formation of the Spliceosomal B complex

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Formation of the Spliceosomal B complex Show undefined attributes

authored	[InstanceEdit:9769907] May, Bruce, 2022-03-22
catalystActivity	[CatalystActivity:9770929] RNA helicase activity of Spliceosomal Pre-B complex [nucleoplasm]
category	uncertain
compartment	[Compartment:7660] nucleoplasm
created	[InstanceEdit:9770146] May, Bruce, 2022-03-24
dbId	9770142
deleted	[Deleted:9830324] Deletion of instance: 72127
displayName	Formation of the Spliceosomal B complex
edited	[InstanceEdit:9769907] May, Bruce, 2022-03-22
eventOf	[Pathway:R-HSA-72163] mRNA Splicing - Major Pathway - Homo sapiens
followingEvent	[BlackBoxEvent:R-HSA-9770145] Formation of the Spliceosomal Bact complex - Homo sapiens
input	[DefinedSet:R-HSA-9770781] ASAP complex, PSAP complex [nucleoplasm] [SimpleEntity:R-ALL-29358] ATP [nucleoplasm] [EntityWithAccessionedSequence:R-HSA-2396419] MFAP1 [nucleoplasm] [EntityWithAccessionedSequence:R-HSA-8867889] PQBP1 [nucleoplasm] [EntityWithAccessionedSequence:R-HSA-8867897] PRPF38A [nucleoplasm] [Complex:R-HSA-9769523] RES complex [nucleoplasm] [Complex:R-HSA-9769528] Spliceosomal Pre-B complex [nucleoplasm] [EntityWithAccessionedSequence:R-HSA-9770255] UBL5 [nucleoplasm] [EntityWithAccessionedSequence:R-HSA-8867887] WBP11 [nucleoplasm] [EntityWithAccessionedSequence:R-HSA-8867895] WBP4 [nucleoplasm] [EntityWithAccessionedSequence:R-HSA-9770248] ZMAT2 [nucleoplasm]
isInDisease	false
isInferred	false
literatureReference	[LiteratureReference:72117] Large-scale proteomic analysis of the human spliceosome. [LiteratureReference:5566502] Protein composition and electron microscopy structure of affinity-purified human spliceosomal B complexes isolated under physiological conditions [LiteratureReference:9769583] Structural Insights into Nuclear pre-mRNA Splicing in Higher Eukaryotes [LiteratureReference:9769580] Structure of the human activated spliceosome in three conformational states [LiteratureReference:6806809] Characterization of purified human Bact spliceosomal complexes reveals compositional and morphological changes during spliceosome activation and first step catalysis [LiteratureReference:9770574] Mass spectrometry-based relative quantification of proteins in precatalytic and catalytically active spliceosomes by metabolic labeling (SILAC), chemical labeling (iTRAQ), and label-free spectral count [LiteratureReference:8865916] Isolation of an active step I spliceosome and composition of its RNP core [LiteratureReference:9772624] Cryo-EM Structure of a Pre-catalytic Human Spliceosome Primed for Activation [LiteratureReference:9770241] A spliceosome intermediate with loosely associated tri-snRNP accumulates in the absence of Prp28 ATPase activity [LiteratureReference:9772622] Structural and functional analysis of the human spliceosomal DEAD-box helicase Prp28 [LiteratureReference:9772614] The 100-kda U5 snRNP protein (hPrp28p) contacts the 5' splice site through its ATPase site [LiteratureReference:9772724] Exon, intron and splice site locations in the spliceosomal B complex [LiteratureReference:9818098] Stable tri-snRNP integration is accompanied by a major structural rearrangement of the spliceosome that is dependent on Prp8 interaction with the 5' splice site [LiteratureReference:9769531] Mechanism of 5' splice site transfer for human spliceosome activation
maxDepth	1
modified	[InstanceEdit:9984900] Weiser, Joel, 2026-03-16
name	Formation of the Spliceosomal B complex
orthologousEvent	[BlackBoxEvent:R-MMU-9770142] Formation of the Spliceosomal B complex - Mus musculus
output	[SimpleEntity:R-ALL-113582] ADP [nucleoplasm] [EntityWithAccessionedSequence:R-HSA-71879] DDX23 [nucleoplasm] [SimpleEntity:R-ALL-113550] Pi [nucleoplasm] [EntityWithAccessionedSequence:R-HSA-8867890] PRPF40A [nucleoplasm] [EntityWithAccessionedSequence:R-HSA-9769525] PRPF4B [nucleoplasm] [EntityWithAccessionedSequence:R-HSA-9770250] RBM25 [nucleoplasm] [EntityWithAccessionedSequence:R-HSA-9770254] RBM39 [nucleoplasm] [EntityWithAccessionedSequence:R-HSA-9769526] RBM42 [nucleoplasm] [EntityWithAccessionedSequence:R-HSA-72049] RBM5 [nucleoplasm] [EntityWithAccessionedSequence:R-HSA-4615999] SART1 [nucleoplasm] [EntityWithAccessionedSequence:R-HSA-6806778] SNRNP27 [nucleoplasm] [Complex:R-HSA-9770126] Spliceosomal B complex [nucleoplasm] [Complex:R-HSA-71917] U1 snRNP [nucleoplasm] [EntityWithAccessionedSequence:R-HSA-6806793] USP39 [nucleoplasm]
precedingEvent	[BlackBoxEvent:R-HSA-9770132] Formation of the Spliceosomal Pre-B complex - Homo sapiens
releaseDate	2022-12-07
reviewStatus	[ReviewStatus:9821382] five stars
reviewed	[InstanceEdit:9818818] Charenton, Cl�ment, 2022-10-20
schemaClass	BlackBoxEvent
species	[Species:48887] Homo sapiens
stId	R-HSA-9770142
summation	[Summation:9771074] The DDX23 RNA helicase (homolog of yeast Prp28) binds ATP to...

Referrals

(precedingEvent)	[BlackBoxEvent:R-HSA-9770145] Formation of the Spliceosomal Bact complex
(hasEvent)	[Pathway:R-HSA-72163] mRNA Splicing - Major Pathway
(replacementInstances)	[Deleted:9830324] Deletion of instance: 72127

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