Reactome | Postmitotic nuclear pore complex (NPC) reformation

Postmitotic nuclear pore complex (NPC) reformation

Stable Identifier

R-HSA-9615933

Type

Pathway

Species

Homo sapiens

ReviewStatus

5/5

Locations in the PathwayBrowser

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Cell Cycle (Homo sapiens)

- Cell Cycle, Mitotic (Homo sapiens)
  - M Phase (Homo sapiens)
    - Mitotic Metaphase and Anaphase (Homo sapiens)
      - Mitotic Anaphase (Homo sapiens)
        
        Nuclear Envelope (NE) Reassembly (Homo sapiens)
        
        Postmitotic nuclear pore complex (NPC) reformation (Homo sapiens)

General

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Postmitotic nuclear pore complex (NPC) reformation

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The NPC is reassembled during late anaphase/telophase when nascent nuclear membranes associate with the chromatin surfaces (reviewed by Wandke and Kutay 2013). Assembly of specific NPC proteins (nucleoporins) into the reforming NPC occurs in a temporally ordered fashion (reviewed by Otsuka and Ellenberg 2018). The GTPase RAN plays a central role in regulating NPC assembly during telophase, as well as earlier events in mitosis, such as mitotic spindle assembly (reviewed by Zierhut and Funabiki 2015). The active form of RAN (RAN:GTP), which is generated by the chromatin-associated RAN guanine nucleotide exchange factor RCC1, is converted to the inactive form (RAN:GDP) by the cytoplasmically localized RAN GTPase activating protein RANGAP1. During telophase, the elevated RAN:GTP near chromatin releases nucleoporins from complexes with nuclear transport receptors, including KPNB1/KPNA (importin alpha/beta) and TPNO1 (transportin), thereby liberating the nucleoporins for NPC assembly (reviewed by Forbes et al. 2015).

Literature References

PubMed ID	Title	Journal	Year
25982429	Nuclear transport factors: global regulation of mitosis Travesa, A, Nord, MS, Forbes, DJ, Bernis, C	Curr. Opin. Cell Biol.	2015
29119545	Mechanisms of nuclear pore complex assembly - two different ways of building one molecular machine Otsuka, S, Ellenberg, J	FEBS Lett.	2018
23498932	Enclosing chromatin: reassembly of the nucleus after open mitosis Wandke, C, Kutay, U	Cell	2013
26222742	Nucleosome functions in spindle assembly and nuclear envelope formation Funabiki, H, Zierhut, C	Bioessays	2015