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Schema
>
Reaction
>
Entries
aspartate + glutamine + ATP <=> asparagine + glutamate + AMP + pyrophosphate [ASNS]
Show undefined attributes
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catalystActivity
[CatalystActivity:70598] asparagine synthase (glutamine-hydrolyzing) activity of ASNS dimer [cytosol]
catalystActivityReference
[CatalystActivityReference:9643819] asparagine synthase (glutamine-hydrolyzing) activity of ASNS dimer [cytosol] Expression of human asparagine synthetase in Escherichia coli
category
transition
compartment
[Compartment:70101] cytosol
crossReference
[DatabaseIdentifier:12667529] RHEA:12229
[DatabaseIdentifier:9925769] RHEA:12229
dbId
70599
displayName
aspartate + glutamine + ATP <=> asparagine + glutamate + AMP + pyrophosphate [ASNS]
eventOf
[Pathway:R-HSA-8963693] Aspartate and asparagine metabolism - Homo sapiens
followingEvent
[Reaction:R-HSA-71732] PPA1 hydrolyzes pyrophosphate to orthophosphate - Homo sapiens
input
[SimpleEntity:R-ALL-113592] ATP [cytosol]
[SimpleEntity:R-ALL-29356] H2O [cytosol]
[SimpleEntity:R-ALL-29448] L-Asp [cytosol]
[SimpleEntity:R-ALL-29472] L-Gln [cytosol]
isInDisease
false
isInferred
false
literatureReference
[LiteratureReference:199780] Expression of human asparagine synthetase in Escherichia coli
maxDepth
1
modified
[InstanceEdit:11108703] Weiser, Joel, 2026-03-16
name
aspartate + glutamine + ATP <=> asparagine + glutamate + AMP + pyrophosphate [ASNS]
ATP + H2O + L-aspartate + L-glutamine => AMP + diphosphate + H(+) + L-asparagine + L-glutamate
negativePrecedingEvent
[NegativePrecedingEvent:9930400] Futile Cycle: ASPG hydrolyses L-Asn to L-Asp
[NegativePrecedingEvent:9930395] Futile Cycle: SLC25A12,13 exchange L-Glu and L-Asp
[NegativePrecedingEvent:9930380] Futile Cycle: oxaloacetate + glutamate <=> aspartate + alpha-ketoglutarate [GOT1]
orthologousEvent
[Reaction:R-PFA-70599] aspartate + glutamine + ATP <=> asparagine + glutamate + AMP + pyrophosphate [ASNS] - Plasmodium falciparum
[Reaction:R-SCE-70599] aspartate + glutamine + ATP <=> asparagine + glutamate + AMP + pyrophosphate [ASNS] - Saccharomyces cerevisiae
[Reaction:R-SPO-70599] aspartate + glutamine + ATP <=> asparagine + glutamate + AMP + pyrophosphate [ASNS] - Schizosaccharomyces pombe
[Reaction:R-DDI-70599] aspartate + glutamine + ATP <=> asparagine + glutamate + AMP + pyrophosphate [ASNS] - Dictyostelium discoideum
[Reaction:R-CEL-70599] aspartate + glutamine + ATP <=> asparagine + glutamate + AMP + pyrophosphate [ASNS] - Caenorhabditis elegans
[Reaction:R-GGA-70599] aspartate + glutamine + ATP <=> asparagine + glutamate + AMP + pyrophosphate [ASNS] - Gallus gallus
[Reaction:R-XTR-70599] aspartate + glutamine + ATP <=> asparagine + glutamate + AMP + pyrophosphate [ASNS] - Xenopus tropicalis
[Reaction:R-DRE-70599] aspartate + glutamine + ATP <=> asparagine + glutamate + AMP + pyrophosphate [ASNS] - Danio rerio
[Reaction:R-SSC-70599] aspartate + glutamine + ATP <=> asparagine + glutamate + AMP + pyrophosphate [ASNS] - Sus scrofa
[Reaction:R-BTA-70599] aspartate + glutamine + ATP <=> asparagine + glutamate + AMP + pyrophosphate [ASNS] - Bos taurus
[Reaction:R-CFA-70599] aspartate + glutamine + ATP <=> asparagine + glutamate + AMP + pyrophosphate [ASNS] - Canis familiaris
[Reaction:R-RNO-70599] aspartate + glutamine + ATP <=> asparagine + glutamate + AMP + pyrophosphate [ASNS] - Rattus norvegicus
[Reaction:R-MMU-70599] aspartate + glutamine + ATP <=> asparagine + glutamate + AMP + pyrophosphate [ASNS] - Mus musculus
output
[SimpleEntity:R-ALL-76577] AMP [cytosol]
[SimpleEntity:R-ALL-70106] H+ [cytosol]
[SimpleEntity:R-ALL-29642] L-Asn [cytosol]
[SimpleEntity:R-ALL-29404] L-Glu [cytosol]
[SimpleEntity:R-ALL-111294] PPi [cytosol]
precedingEvent
[Reaction:R-HSA-5691507] ASPA deacetylates NAASP to acetate and L-aspartate - Homo sapiens
previousReviewStatus
[ReviewStatus:9821385] four stars
releaseDate
2004-07-06
reviewStatus
[ReviewStatus:9821382] five stars
reviewed
[InstanceEdit:9927660] D'Eustachio, Peter, 2024-11-08
schemaClass
Reaction
species
[Species:48887] Homo sapiens
stId
R-HSA-70599
summation
[Summation:178479] Cytosolic asparagine synthase (ASNS) catalyzes the reaction ...
updateTrackers
[UpdateTracker:9792362] Update Tracker - [Reaction:70599] aspartate + glutamine + ATP <=> asparagine + glutamate + AMP + pyrophosphate [ASNS] - v22:[addLiteratureReference]
[UpdateTracker:9928488] Update Tracker - [Reaction:70599] aspartate + glutamine + ATP <=> asparagine + glutamate + AMP + pyrophosphate [ASNS] - v91:[removeOutput]
[UpdateTracker:9790405] Update Tracker - [Reaction:70599] aspartate + glutamine + ATP <=> asparagine + glutamate + AMP + pyrophosphate [ASNS] - v33:[addInput]
[UpdateTracker:9790963] Update Tracker - [Reaction:70599] aspartate + glutamine + ATP <=> asparagine + glutamate + AMP + pyrophosphate [ASNS] - v32:[modifyText]
Referrals
(updatedInstance)
[UpdateTracker:9792362] Update Tracker - [Reaction:70599] aspartate + glutamine + ATP <=> asparagine + glutamate + AMP + pyrophosphate [ASNS] - v22:[addLiteratureReference]
[UpdateTracker:9928488] Update Tracker - [Reaction:70599] aspartate + glutamine + ATP <=> asparagine + glutamate + AMP + pyrophosphate [ASNS] - v91:[removeOutput]
[UpdateTracker:9790405] Update Tracker - [Reaction:70599] aspartate + glutamine + ATP <=> asparagine + glutamate + AMP + pyrophosphate [ASNS] - v33:[addInput]
[UpdateTracker:9790963] Update Tracker - [Reaction:70599] aspartate + glutamine + ATP <=> asparagine + glutamate + AMP + pyrophosphate [ASNS] - v32:[modifyText]
(precedingEvent)
[Reaction:R-HSA-71732] PPA1 hydrolyzes pyrophosphate to orthophosphate
[NegativePrecedingEvent:9930377] Futile Cycle: aspartate + glutamine + ATP <=> asparagine + glutamate + AMP + pyrophosphate [ASNS]
[NegativePrecedingEvent:9930398] Futile Cycle: aspartate + glutamine + ATP <=> asparagine + glutamate + AMP + pyrophosphate [ASNS]
[NegativePrecedingEvent:9930396] Futile Cycle: aspartate + glutamine + ATP <=> asparagine + glutamate + AMP + pyrophosphate [ASNS]
(hasEvent)
[Pathway:R-HSA-8963693] Aspartate and asparagine metabolism
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