Reactome | Nonhomologous End-Joining (NHEJ)

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Nonhomologous End-Joining (NHEJ) Show undefined attributes

authored	[InstanceEdit:73967] Lees-Miller, S, 2003-07-14 15:03:25
compartment	[Compartment:7660] nucleoplasm
created	[InstanceEdit:5693618] Orlic-Milacic, Marija, 2015-05-16
dbId	5693571
deleted	[Deleted:6793533] Deletion of instance: 73889
displayName	Nonhomologous End-Joining (NHEJ)
edited	[InstanceEdit:165073] Joshi-Tope, G, 0000-00-00 00:00:00 [InstanceEdit:5692524] Orlic-Milacic, Marija, 2015-05-12
eventOf	[Pathway:R-HSA-5693532] DNA Double-Strand Break Repair - Homo sapiens
figure	[Figure:77104] /figures/NHEJFig60dpi.jpg
goBiologicalProcess	[GO_BiologicalProcess:17665] double-strand break repair via nonhomologous end joining
hasDiagram	true
hasEHLD	false
hasEvent	[Reaction:R-HSA-5686685] RIF1 and PAX1IP bind TP53BP1 at DNA DSBs - Homo sapiens [Reaction:R-HSA-5693599] Association of Ku heterodimer with ends of DNA double-strand break - Homo sapiens [Reaction:R-HSA-5693615] Association of DNA-PKcs with Ku-bound ends of DNA double-strand breaks - synapsis - Homo sapiens [Reaction:R-HSA-5686900] TP53BP1 recruits DCLRE1C to ATM - Homo sapiens [Reaction:R-HSA-5686704] Activated ATM phosphorylates DCLRE1C - Homo sapiens [Reaction:R-HSA-5693575] DNA-PKcs autophosphorylates - Homo sapiens [Reaction:R-HSA-5686924] DCLRE1C binds PRKDC:XRCC5:XRCC6 at DNA DSBs - Homo sapiens [Reaction:R-HSA-5687183] PRKDC phosphorylates DCLRE1C at DNA DSBs - Homo sapiens [Reaction:R-HSA-5693533] DCLRE1C (ARTEMIS) processes DNA DSB ends - Homo sapiens [Reaction:R-HSA-5693578] TDP1 and TDP2 process unligatable DSB ends - Homo sapiens [Reaction:R-HSA-5693574] XRCC4:LIG4, NHEJ1 and POLL or POLM bind DNA DSBs in NHEJ - Homo sapiens [Reaction:R-HSA-5687360] POLL or POLM extends aligned DNA DSB ends to fill gaps - Homo sapiens [Reaction:R-HSA-5693604] XRCC4:LIG4 ligates DNA DSB ends during NHEJ - Homo sapiens
isInDisease	false
isInferred	false
lastUpdatedDate	2022-04-07
literatureReference	[LiteratureReference:76312] Binding of inositol phosphate to DNA-PK and stimulation of double-strand break repair. [LiteratureReference:912403] ATM activation by DNA double-strand breaks through the Mre11-Rad50-Nbs1 complex [LiteratureReference:83547] A critical role for histone H2AX in recruitment of repair factors to nuclear foci after DNA damage. [LiteratureReference:5682158] ATM-dependent phosphorylation of nibrin in response to radiation exposure [LiteratureReference:5682186] MDC1 directly binds phosphorylated histone H2AX to regulate cellular responses to DNA double-strand breaks [LiteratureReference:83562] MDC1 is a mediator of the mammalian DNA damage checkpoint. [LiteratureReference:5682988] RNF8- and RNF168-dependent degradation of KDM4A/JMJD2A triggers 53BP1 recruitment to DNA damage sites [LiteratureReference:5682980] MMSET regulates histone H4K20 methylation and 53BP1 accumulation at DNA damage sites [LiteratureReference:5683382] Abraxas and RAP80 form a BRCA1 protein complex required for the DNA damage response [LiteratureReference:110405] 53BP1, a mediator of the DNA damage checkpoint. [LiteratureReference:5683732] NFBD1/MDC1, 53BP1 and BRCA1 have both redundant and unique roles in the ATM pathway [LiteratureReference:5686687] A cell cycle-dependent regulatory circuit composed of 53BP1-RIF1 and BRCA1-CtIP controls DNA repair pathway choice [LiteratureReference:5686691] 53BP1 regulates DSB repair using Rif1 to control 5' end resection [LiteratureReference:5686906] 53BP1 mediates productive and mutagenic DNA repair through distinct phosphoprotein interactions [LiteratureReference:5686707] A pathway of double-strand break rejoining dependent upon ATM, Artemis, and proteins locating to gamma-H2AX foci [LiteratureReference:5686902] PTIP associates with Artemis to dictate DNA repair pathway choice [LiteratureReference:76260] Structure of the Ku heterodimer bound to DNA and its implications for double-strand break repair. [LiteratureReference:5686698] Human Ku70/80 protein blocks exonuclease 1-mediated DNA resection in the presence of human Mre11 or Mre11/Rad50 protein complex [LiteratureReference:76264] The DNA-dependent protein kinase: requirement for DNA ends and association with Ku antigen. [LiteratureReference:76267] Geometry of a complex formed by double strand break repair proteins at a single DNA end: recruitment of DNA-PKcs induces inward translocation of Ku protein. [LiteratureReference:76272] Hairpin opening and overhang processing by an Artemis/DNA-dependent protein kinase complex in nonhomologous end joining and V(D)J recombination. [LiteratureReference:5687198] The DNA-dependent protein kinase catalytic subunit phosphorylation sites in human Artemis [LiteratureReference:5687216] DNA-PKcs dependence of Artemis endonucleolytic activity, differences between hairpins and 5' or 3' overhangs [LiteratureReference:175594] Conversion of phosphoglycolate to phosphate termini on 3' overhangs of DNA double strand breaks by the human tyrosyl-DNA phosphodiesterase hTdp1 [LiteratureReference:5687398] TDP2-dependent non-homologous end-joining protects against topoisomerase II-induced DNA breaks and genome instability in cells and in vivo [LiteratureReference:5687191] Ku recruits the XRCC4-ligase IV complex to DNA ends [LiteratureReference:5687201] Defining interactions between DNA-PK and ligase IV/XRCC4 [LiteratureReference:5687167] Artemis C-terminal region facilitates V(D)J recombination through its interactions with DNA Ligase IV and DNA-PKcs [LiteratureReference:5687240] XLF interacts with the XRCC4-DNA ligase IV complex to promote DNA nonhomologous end-joining [LiteratureReference:5687244] Cernunnos/XLF promotes the ligation of mismatched and noncohesive DNA ends [LiteratureReference:5687410] Association of DNA polymerase mu (pol mu) with Ku and ligase IV: role for pol mu in end-joining double-strand break repair [LiteratureReference:5687415] Implication of DNA polymerase lambda in alignment-based gap filling for nonhomologous DNA end joining in human nuclear extracts [LiteratureReference:5687412] DNA polymerase lambda can elongate on DNA substrates mimicking non-homologous end joining and interact with XRCC4-ligase IV complex
maxDepth	2
modified	[InstanceEdit:10996769] Weiser, Joel, 2026-03-16
name	Nonhomologous End-Joining (NHEJ)
orthologousEvent	[Pathway:R-DDI-5693571] Nonhomologous End-Joining (NHEJ) - Dictyostelium discoideum [Pathway:R-RNO-5693571] Nonhomologous End-Joining (NHEJ) - Rattus norvegicus [Pathway:R-CFA-5693571] Nonhomologous End-Joining (NHEJ) - Canis familiaris [Pathway:R-BTA-5693571] Nonhomologous End-Joining (NHEJ) - Bos taurus [Pathway:R-SSC-5693571] Nonhomologous End-Joining (NHEJ) - Sus scrofa [Pathway:R-DRE-5693571] Nonhomologous End-Joining (NHEJ) - Danio rerio [Pathway:R-XTR-5693571] Nonhomologous End-Joining (NHEJ) - Xenopus tropicalis [Pathway:R-GGA-5693571] Nonhomologous End-Joining (NHEJ) - Gallus gallus [Pathway:R-MMU-5693571] Nonhomologous End-Joining (NHEJ) - Mus musculus
precedingEvent	[Pathway:R-HSA-5693606] DNA Double Strand Break Response - Homo sapiens
releaseDate	2003-11-03
reviewStatus	[ReviewStatus:9821382] five stars
reviewed	[InstanceEdit:165095] West, SC, 0000-00-00 00:00:00 [InstanceEdit:5693179] Borowiec, James A, 2015-06-12
revised	[InstanceEdit:5692524] Orlic-Milacic, Marija, 2015-05-12
schemaClass	Pathway
species	[Species:48887] Homo sapiens
stId	R-HSA-5693571
summation	[Summation:5693611] The nonhomologous end joining (NHEJ) pathway is initiated in...
updateTrackers	[UpdateTracker:9776514] Update Tracker - [Pathway:5693571] Nonhomologous End-Joining (NHEJ) - v75:[updateContainedRLE] [UpdateTracker:9775071] Update Tracker - [Pathway:5693571] Nonhomologous End-Joining (NHEJ) - v80:[updateContainedRLE]

Referrals

(hasEvent)	[Pathway:R-HSA-5693532] DNA Double-Strand Break Repair
(hasEncapsulatedEvent)	[Pathway:R-HSA-5693567] HDR through Homologous Recombination (HRR) or Single Strand Annealing (SSA) [Pathway:R-HSA-5693606] DNA Double Strand Break Response
(replacementInstances)	[Deleted:6793533] Deletion of instance: 73889
(updatedInstance)	[UpdateTracker:9776514] Update Tracker - [Pathway:5693571] Nonhomologous End-Joining (NHEJ) - v75:[updateContainedRLE] [UpdateTracker:9775071] Update Tracker - [Pathway:5693571] Nonhomologous End-Joining (NHEJ) - v80:[updateContainedRLE]

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