Reactome | eNOS synthesizes NO

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eNOS synthesizes NO Show undefined attributes

authored	[InstanceEdit:202104] Hemish, J, 2007-10-19 18:00:42
catalystActivity	[CatalystActivity:203600] nitric-oxide synthase activity of p-S1177-eNOS:CaM:HSP90:p-AKT1:BH4 [plasma membrane]
catalystActivityReference	[CatalystActivityReference:9911158] nitric-oxide synthase activity of p-S1177-eNOS:CaM:HSP90:p-AKT1:BH4 [plasma membrane] Cloning and expression of a cDNA encoding human endothelium-derived relaxing factor/nitric oxide synthase
category	transition
compartment	[Compartment:70101] cytosol [Compartment:876] plasma membrane
created	[InstanceEdit:202104] Hemish, J, 2007-10-19 18:00:42
crossReference	[DatabaseIdentifier:9911125] RHEA:19898
dbId	202127
displayName	eNOS synthesizes NO
eventOf	[Pathway:R-HSA-203615] eNOS activation - Homo sapiens [Pathway:R-HSA-9009391] Extra-nuclear estrogen signaling - Homo sapiens [Pathway:R-HSA-5218920] VEGFR2 mediated vascular permeability - Homo sapiens
followingEvent	[Reaction:R-HSA-1497878] Superoxide reacts rapidly with NO to form peroxynitrite (ONOO-) - Homo sapiens
input	[SimpleEntity:R-ALL-70106] H+ [cytosol] 2 × [SimpleEntity:R-ALL-29468] L-Arg [cytosol] 3 × [SimpleEntity:R-ALL-29364] NADPH [cytosol] [SimpleEntity:R-ALL-29368] O2 [cytosol]
isInDisease	false
isInferred	false
literatureReference	[LiteratureReference:202106] Nitric oxide: a physiologic messenger molecule [LiteratureReference:202120] Nitric Oxide as a Unique Bioactive Signaling Messenger in Physiology and Pathophysiology [LiteratureReference:203616] Determination of nitric oxide synthase cofactors: heme, FAD, FMN, and tetrahydrobiopterin [LiteratureReference:203569] Characterization of bovine endothelial nitric oxide synthase as a homodimer with down-regulated uncoupled NADPH oxidase activity: tetrahydrobiopterin binding kinetics and role of haem in dimerization [LiteratureReference:9911175] Cloning and expression of a cDNA encoding human endothelium-derived relaxing factor/nitric oxide synthase
maxDepth	1
modified	[InstanceEdit:11080360] Weiser, Joel, 2026-03-16
name	eNOS synthesizes NO H(+) + 2 L-arginine + 3 NADPH + 4 O2 => 4 H2O + 2 L-citrulline + 3 NADP(+) + 2 nitric oxide NO biosynthesis
orthologousEvent	[Reaction:R-SCE-202127] eNOS synthesizes NO - Saccharomyces cerevisiae [Reaction:R-SPO-202127] eNOS synthesizes NO - Schizosaccharomyces pombe [Reaction:R-DDI-202127] eNOS synthesizes NO - Dictyostelium discoideum [Reaction:R-CEL-202127] eNOS synthesizes NO - Caenorhabditis elegans [Reaction:R-DME-202127] eNOS synthesizes NO - Drosophila melanogaster [Reaction:R-BTA-202127] eNOS synthesizes NO - Bos taurus [Reaction:R-RNO-202127] eNOS synthesizes NO - Rattus norvegicus [Reaction:R-MMU-202127] eNOS synthesizes NO - Mus musculus
output	4 × [SimpleEntity:R-ALL-29356] H2O [cytosol] 2 × [SimpleEntity:R-ALL-29968] L-Cit [cytosol] 3 × [SimpleEntity:R-ALL-29366] NADP+ [cytosol] 2 × [SimpleEntity:R-ALL-202124] NO [cytosol]
precedingEvent	[Reaction:R-HSA-1497784] The cofactor BH4 is required for electron transfer in the eNOS catalytic cycle - Homo sapiens
previousReviewStatus	[ReviewStatus:9821385] four stars
regulatedBy	[NegativeRegulation:R-HSA-6783968] Negative regulation by 'ADMA [cytosol]'
releaseDate	2008-03-12
reviewStatus	[ReviewStatus:9821382] five stars
reviewed	[InstanceEdit:215470] Enikolopov, G, 2008-02-28 17:52:44 [InstanceEdit:9917333] Hill, David P, 2024-08-02
schemaClass	Reaction
species	[Species:48887] Homo sapiens
stId	R-HSA-202127
summation	[Summation:202119] Nitric oxide (NO) is produced from L-arginine by the family ...
updateTrackers	[UpdateTracker:9784171] Update Tracker - [Reaction:202127] eNOS synthesizes NO - v54:[addRegulator] [UpdateTracker:9789460] Update Tracker - [Reaction:202127] eNOS synthesizes NO - v38:[modifyText] [UpdateTracker:9919399] Update Tracker - [Reaction:202127] eNOS synthesizes NO - v90:[modifyText, removeInput, removeLiteratureReference, removeOutput]

Referrals

(updatedInstance)	[UpdateTracker:9784171] Update Tracker - [Reaction:202127] eNOS synthesizes NO - v54:[addRegulator] [UpdateTracker:9789460] Update Tracker - [Reaction:202127] eNOS synthesizes NO - v38:[modifyText] [UpdateTracker:9919399] Update Tracker - [Reaction:202127] eNOS synthesizes NO - v90:[modifyText, removeInput, removeLiteratureReference, removeOutput]
(hasEvent)	[Pathway:R-HSA-9009391] Extra-nuclear estrogen signaling [Pathway:R-HSA-5218920] VEGFR2 mediated vascular permeability [Pathway:R-HSA-203615] eNOS activation
(precedingEvent)	[Reaction:R-HSA-1497878] Superoxide reacts rapidly with NO to form peroxynitrite (ONOO-)

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