Reactome | eNOS synthesizes NO

eNOS synthesizes NO

Stable Identifier

R-HSA-202127

Type

Reaction [transition]

Species

Homo sapiens

Compartment

cytosol, plasma membrane

Synonyms

H(+) + 2 L-arginine + 3 NADPH + 4 O2 => 4 H2O + 2 L-citrulline + 3 NADP(+) + 2 nitric oxide, NO biosynthesis

ReviewStatus

5/5

Locations in the PathwayBrowser

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Signal Transduction (Homo sapiens)

- Signaling by Nuclear Receptors (Homo sapiens)
  - ESR-mediated signaling (Homo sapiens)
    - Extra-nuclear estrogen signaling (Homo sapiens)
      - eNOS synthesizes NO (Homo sapiens)
- Signaling by Receptor Tyrosine Kinases (Homo sapiens)
  - Signaling by VEGF (Homo sapiens)
    - VEGFA-VEGFR2 Pathway (Homo sapiens)
      - VEGFR2 mediated vascular permeability (Homo sapiens)
        
        eNOS synthesizes NO (Homo sapiens)

General

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Nitric oxide (NO) is produced from L-arginine by the family of nitric oxide synthases (NOS) enzymes, forming the free radical NO and citrulline as byproduct (Janssens et al. 1992). The cofactor tetrahydrobiopterin (BH4) is an essential requirement for the delivery of an electron to the intermediate in the catalytic cycle of NOS.

Literature References

PubMed ID	Title	Journal	Year
15467163	Nitric Oxide as a Unique Bioactive Signaling Messenger in Physiology and Pathophysiology Tuteja, N, Chandra, M, Tuteja, R, Misra, MK	J Biomed Biotechnol	2004
7526779	Nitric oxide: a physiologic messenger molecule Bredt, DS, Snyder, SH	Annu Rev Biochem	1994
8782602	Determination of nitric oxide synthase cofactors: heme, FAD, FMN, and tetrahydrobiopterin Klatt, P, Schmidt, K, Werner, ER, Mayer, B	Methods Enzymol	1996
9173876	Characterization of bovine endothelial nitric oxide synthase as a homodimer with down-regulated uncoupled NADPH oxidase activity: tetrahydrobiopterin binding kinetics and role of haem in dimerization List, BM, Klosch, B, Volker, C, Gorren, AC, Sessa, WC, Werner, ER, Kukovetz, WR, Schmidt, K, Mayer, B	Biochem J	1997
1378832	Cloning and expression of a cDNA encoding human endothelium-derived relaxing factor/nitric oxide synthase Janssens, SP, Shimouchi, A, Quertermous, T, Bloch, DB, Bloch, KD	J Biol Chem	1992