Reactome | eIF2:GTP is hydrolyzed, eIFs are released

eIF2:GTP is hydrolyzed, eIFs are released

Stable Identifier

R-HSA-72619

Type

Reaction [dissociation]

Species

Homo sapiens

Compartment

cytosol

ReviewStatus

5/5

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eIF2:GTP is hydrolyzed, eIFs are released

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Once the Met-tRNAi has recognized the AUG, eIF2-bound GTP is hydrolyzed. The reaction is catalyzed by eIF5 (or eIF5B) and is thought to cause dissociation of all other initiation factors and allow joining of the large 60S ribosomal subunit. Release of the initiation factors from 40S leaves the Met-tRNAi in the ribosomal P-site base-paired to the start codon on the mRNA.

Literature References

PubMed ID	Title	Journal	Year
592399	Initiation of mammalian protein synthesis. II. The assembly of the initiation complex with purified initiation factors. Trachsel, H, Erni, B, Schreier, MH, Staehelin, T	J Mol Biol	1978
641056	The mechanism of action of protein synthesis initiation factors from rabbit reticulocytes. Benne, R, Hershey, JW	J Biol Chem	1978
10659855	The joining of ribosomal subunits in eukaryotes requires eIF5B. Pestova, TV, Lomakin, IB, Lee, JH, Choi, SK, Dever, TE, Hellen, CU	Nature	2000
1856230	Function of eukaryotic initiation factor 5 in the formation of an 80 S ribosomal polypeptide chain initiation complex. Chakrabarti, A, Maitra, U	J Biol Chem	1991
11018020	A multifactor complex of eukaryotic initiation factors, eIF1, eIF2, eIF3, eIF5, and initiator tRNA(Met) is an important translation initiation intermediate in vivo. Asano, K, Clayton, J, Shalev, A, Hinnebusch, AG	Genes Dev	2000
429297	Binding and release of radiolabeled eukaryotic initiation factors 2 and 3 during 80 S initiation complex formation. Peterson, DT, Merrick, WC, Safer, B	J Biol Chem	1979