Reactome | PARP1,PARP2 dimers and FEN1 bind POLB and displace APEX1 from damaged AP site

PARP1,PARP2 dimers and FEN1 bind POLB and displace APEX1 from damaged AP site

Stable Identifier

R-HSA-5649873

Type

Reaction [binding]

Species

Homo sapiens

Compartment

nucleoplasm

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PARP1,PARP2 dimers and FEN1 bind POLB and displace APEX1 from damaged AP site

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Homodimers and/or heterodimers of PARP1 and PARP2 bind single strand DNA (ssDNA) ends along with FEN1 (flap endonuclease), forming a ternary complex with POLB (DNA polymerase beta) and simultaneously displacing APEX1 (Prasad et al. 2001, Lavrik et al. 2001, Cistulli et al. 2004). While PARP2 is much less catalytically active than PARP1 in DNA damage-induced poly(ADP-ribosyl) (PAR) synthesis (Shieh et al. 1998, Ame et al. 1999, Fisher et al. 2007), the functional redundancy between PARP1 and PARP2 is probably important. Knockout of both PARP1 and PARP2 homologs is embryonic lethal in mice, while knockout of individual PARPs is not (Menissier de Murcia et al. 2003).

Literature References

PubMed ID	Title	Journal	Year
9804757	Poly(ADP-ribose) polymerase null mouse cells synthesize ADP-ribose polymers Shieh, WM, Wilson, MV, Amé, JC, Jacobson, EL, Jacobson, MK, Wang, ZQ, Koh, DW	J. Biol. Chem.	1998
10364231	PARP-2, A novel mammalian DNA damage-dependent poly(ADP-ribose) polymerase Decker, P, Amé, JC, Schreiber, V, Muller, S, Rolli, V, Apiou, F, de Murcia, G, Ménissier-de Murcia, J, Niedergang, C, Höger, T	J. Biol. Chem.	1999
11340072	Photoaffinity labeling of mouse fibroblast enzymes by a base excision repair intermediate. Evidence for the role of poly(ADP-ribose) polymerase-1 in DNA repair Wilson, SH, Ackerman, EJ, Sobol, RW, Horton, JK, Lavrik, OI, Prasad, R	J. Biol. Chem.	2001
12727891	Functional interaction between PARP-1 and PARP-2 in chromosome stability and embryonic development in mouse de Murcia, G, Chambon, P, Amé, JC, Sabatier, L, Niedergang, C, Ricoul, M, Dantzer, F, Schreiber, V, Dierich, A, LeMeur, M, Ménissier de Murcia, J, Tartier, L, Huber, A	EMBO J.	2003
15135726	AP endonuclease and poly(ADP-ribose) polymerase-1 interact with the same base excision repair intermediate Hou, E, Lavrik, OI, Wilson, SH, Prasad, R, Cistulli, C	DNA Repair (Amst.)	2004
11440997	DNA polymerase beta -mediated long patch base excision repair. Poly(ADP-ribose)polymerase-1 stimulates strand displacement DNA synthesis Vande Berg, BJ, Wilson, SH, Kim, SJ, Kedar, P, Yang, XP, Lavrik, OI, Prasad, R	J. Biol. Chem.	2001
17548475	Poly(ADP-ribose) polymerase 1 accelerates single-strand break repair in concert with poly(ADP-ribose) glycohydrolase Hochegger, H, Takeda, S, Fisher, AE, Caldecott, KW	Mol. Cell. Biol.	2007