Reactome | POLB-Dependent Long Patch Base Excision Repair

POLB-Dependent Long Patch Base Excision Repair

Stable Identifier

R-HSA-110362

Type

Pathway

Species

Homo sapiens

Compartment

nucleoplasm

ReviewStatus

5/5

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During POLB-dependent long patch base excision repair (BER), PARP1 and/or PARP2 is recruited to the BER site along with flap endonuclease FEN1. PARP1 and/or PARP2 and FEN1 facilitate POLB-mediated strand displacement synthesis which involves incorporation of 2-10 nucleotides at the 3' end of the APEX1-created single strand break (SSB). After the DNA strand displacement synthesis is completed and the displaced strand is cleaved, POLB recruits DNA ligase I (LIG1) to ligate the SSB (Klungland and Lindahl 1997, Dimitriadis et al. 1998, Prasad et al. 2001, Lavrik et al. 2001, Tomkinson et al. 2001, Ranalli et al. 2002, Cistulli et al. 2004, Liu et al. 2005).

Literature References

PubMed ID	Title	Journal	Year
15561706	DNA polymerase beta and flap endonuclease 1 enzymatic specificities sustain DNA synthesis for long patch base excision repair Liu, Y, Beard, WA, Shock, DD, Prasad, R, Hou, EW, Wilson, SH	J. Biol. Chem.	2005
11554294	Completion of base excision repair by mammalian DNA ligases Tomkinson, AE, Chen, L, Dong, Z, Leppard, JB, Levin, DS, Mackey, ZB, Motycka, TA	Prog. Nucleic Acid Res. Mol. Biol.	2001
11340072	Photoaffinity labeling of mouse fibroblast enzymes by a base excision repair intermediate. Evidence for the role of poly(ADP-ribose) polymerase-1 in DNA repair Lavrik, OI, Prasad, R, Sobol, RW, Horton, JK, Ackerman, EJ, Wilson, SH	J. Biol. Chem.	2001
12200445	AP endonuclease 1 coordinates flap endonuclease 1 and DNA ligase I activity in long patch base excision repair. Ranalli, TA, Tom, S, Bambara, RA	J Biol Chem	2002
9685411	Thermodynamics of human DNA ligase I trimerization and association with DNA polymerase beta Dimitriadis, EK, Prasad, R, Vaske, MK, Chen, L, Tomkinson, AE, Lewis, MS, Wilson, SH	J. Biol. Chem.	1998
11440997	DNA polymerase beta -mediated long patch base excision repair. Poly(ADP-ribose)polymerase-1 stimulates strand displacement DNA synthesis Prasad, R, Lavrik, OI, Kim, SJ, Kedar, P, Yang, XP, Vande Berg, BJ, Wilson, SH	J. Biol. Chem.	2001
15135726	AP endonuclease and poly(ADP-ribose) polymerase-1 interact with the same base excision repair intermediate Cistulli, C, Lavrik, OI, Prasad, R, Hou, E, Wilson, SH	DNA Repair (Amst.)	2004
9214649	Second pathway for completion of human DNA base excision-repair: reconstitution with purified proteins and requirement for DNase IV (FEN1). Klungland, A, Lindahl, T	EMBO J	1997