POLB-Dependent Long Patch Base Excision Repair

Stable Identifier
Homo sapiens
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During POLB-dependent long patch base excision repair (BER), PARP1 and/or PARP2 is recruited to the BER site along with flap endonuclease FEN1. PARP1 and/or PARP2 and FEN1 facilitate POLB-mediated strand displacement synthesis which involves incorporation of 2-10 nucleotides at the 3' end of the APEX1-created single strand break (SSB). After the DNA strand displacement synthesis is completed and the displaced strand is cleaved, POLB recruits DNA ligase I (LIG1) to ligate the SSB (Klungland and Lindahl 1997, Dimitriadis et al. 1998, Prasad et al. 2001, Lavrik et al. 2001, Tomkinson et al. 2001, Ranalli et al. 2002, Cistulli et al. 2004, Liu et al. 2005).

Literature References
PubMed ID Title Journal Year
15561706 DNA polymerase beta and flap endonuclease 1 enzymatic specificities sustain DNA synthesis for long patch base excision repair

Liu, Y, Beard, WA, Wilson, SH, Prasad, R, Hou, EW, Shock, DD

J. Biol. Chem. 2005
11554294 Completion of base excision repair by mammalian DNA ligases

Tomkinson, AE, Motycka, TA, Mackey, ZB, Levin, DS, Leppard, JB, Chen, L, Dong, Z

Prog. Nucleic Acid Res. Mol. Biol. 2001
11340072 Photoaffinity labeling of mouse fibroblast enzymes by a base excision repair intermediate. Evidence for the role of poly(ADP-ribose) polymerase-1 in DNA repair

Wilson, SH, Ackerman, EJ, Sobol, RW, Horton, JK, Lavrik, OI, Prasad, R

J. Biol. Chem. 2001
12200445 AP endonuclease 1 coordinates flap endonuclease 1 and DNA ligase I activity in long patch base excision repair.

Ranalli, TA, Bambara, RA, Tom, S

J Biol Chem 2002
9685411 Thermodynamics of human DNA ligase I trimerization and association with DNA polymerase beta

Tomkinson, AE, Wilson, SH, Lewis, MS, Vaske, MK, Chen, L, Prasad, R, Dimitriadis, EK

J. Biol. Chem. 1998
11440997 DNA polymerase beta -mediated long patch base excision repair. Poly(ADP-ribose)polymerase-1 stimulates strand displacement DNA synthesis

Vande Berg, BJ, Wilson, SH, Kim, SJ, Kedar, P, Yang, XP, Lavrik, OI, Prasad, R

J. Biol. Chem. 2001
15135726 AP endonuclease and poly(ADP-ribose) polymerase-1 interact with the same base excision repair intermediate

Hou, E, Lavrik, OI, Wilson, SH, Prasad, R, Cistulli, C

DNA Repair (Amst.) 2004
9214649 Second pathway for completion of human DNA base excision-repair: reconstitution with purified proteins and requirement for DNase IV (FEN1).

Lindahl, T, Klungland, A

EMBO J 1997
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