Reactome | Translesion synthesis by REV1

Translesion synthesis by REV1

Stable Identifier

R-HSA-110312

Type

Pathway

Species

Homo sapiens

Compartment

nucleoplasm

ReviewStatus

5/5

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REV1 (hREV1) encodes a template-dependent dCMP transferase that can insert a C residue opposite an abasic site (Lin et al. 1999, Gibbs et al. 2000). Interaction with monoubiquitinated PCNA at a DNA damage site enhances REV1-mediated translesion synthesis (TLS) (Garg and Burgers 2005, Wood et al. 2007). After REV1 incorporates dCMP opposite to the apurinic/apyrimidinic (AP) template site, TLS is continued by the DNA polymerase zeta complex (POLZ). POLZ consists of the catalytic subunit REV3L and the accessory subunit MAD2L2 (REV7). MAD2L2 binds REV1, thus recruiting POLZ to DNA damage site (Hara et al. 2010, Kikuchi et al. 2010, Xie et al. 1012). POLZ is error-prone and contributes to TLS-related mutagenesis (Shachar et al. 2009, Lee et al. 2014). POLZ has a low processivity and dissociates from the DNA template after incorporating less than 30 nucleotides (Nelson et al. 1996, Lee et al. 2014).

Literature References

PubMed ID	Title	Journal	Year
17517887	A ubiquitin-binding motif in the translesion DNA polymerase Rev1 mediates its essential functional interaction with ubiquitinated proliferating cell nuclear antigen in response to DNA damage Wood, A, Garg, P, Burgers, PM	J. Biol. Chem.	2007
23143872	Structural insights into the assembly of human translesion polymerase complexes Xie, W, Yang, X, Xu, M, Jiang, T	Protein Cell	2012
16344468	Ubiquitinated proliferating cell nuclear antigen activates translesion DNA polymerases eta and REV1 Garg, P, Burgers, PM	Proc. Natl. Acad. Sci. U.S.A.	2005
8658138	Thymine-thymine dimer bypass by yeast DNA polymerase zeta Nelson, JR, Lawrence, CW, Hinkle, DC	Science	1996
22859296	Structural basis of recruitment of DNA polymerase ? by interaction between REV1 and REV7 proteins Kikuchi, S, Hara, K, Shimizu, T, Sato, M, Hashimoto, H	J. Biol. Chem.	2012
10536157	The human REV1 gene codes for a DNA template-dependent dCMP transferase. Lin, W, Xin, H, Zhang, Y, Wu, X, Yuan, F	Nucleic Acids Res	1999
20164194	Crystal structure of human REV7 in complex with a human REV3 fragment and structural implication of the interaction between DNA polymerase zeta and REV1 Hara, K, Hashimoto, H, Murakumo, Y, Kobayashi, S, Kogame, T, Unzai, S, Akashi, S, Takeda, S, Shimizu, T, Sato, M	J. Biol. Chem.	2010
24449906	Human Pol ? purified with accessory subunits is active in translesion DNA synthesis and complements Pol ? in cisplatin bypass Lee, YS, Gregory, MT, Yang, W	Proc. Natl. Acad. Sci. U.S.A.	2014
10760286	The function of the human homolog of Saccharomyces cerevisiae REV1 is required for mutagenesis induced by UV light. Gibbs, PE, Wang, XD, Li, Z, McManus, TP, McGregor, WG, Lawrence, CW, Maher, VM	Proc Natl Acad Sci U S A	2000
19153606	Two-polymerase mechanisms dictate error-free and error-prone translesion DNA synthesis in mammals Shachar, S, Ziv, O, Avkin, S, Adar, S, Wittschieben, J, Reissner, T, Chaney, S, Friedberg, EC, Wang, Z, Carell, T, Geacintov, N, Livneh, Z	EMBO J.	2009