REV1 binds AP-dsDNA

Stable Identifier
R-HSA-110307
Type
Reaction [binding]
Species
Homo sapiens
Compartment
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REV1 is a deoxycytidyl transferase that belongs to the DNA polymerase type-Y family. REV1 was cloned as the human homolog of yeast REV1. Similar to its yeast counterpart, REV1 binds damaged DNA, with the preferred substrate being DNA with an AP (abasic - apurinic/apyrimidinic) site. The mechanism for DNA damage recognition has not been elucidated (Lin et al. 1999, Gibbs et al. 2000). Besides DNA binding, REV1 has a ubiquitin binding motif in its C-terminal domain that interacts with monoubiquitinated PCNA, which enhances REV1-mediated translesion synthesis (Garg and Burgers 2005, Wood et al. 2007).

Literature References
PubMed ID Title Journal Year
16344468 Ubiquitinated proliferating cell nuclear antigen activates translesion DNA polymerases eta and REV1

Garg, P, Burgers, PM

Proc. Natl. Acad. Sci. U.S.A. 2005
17517887 A ubiquitin-binding motif in the translesion DNA polymerase Rev1 mediates its essential functional interaction with ubiquitinated proliferating cell nuclear antigen in response to DNA damage

Wood, A, Garg, P, Burgers, PM

J. Biol. Chem. 2007
10536157 The human REV1 gene codes for a DNA template-dependent dCMP transferase.

Lin, W, Xin, H, Zhang, Y, Wu, X, Yuan, F

Nucleic Acids Res 1999
10760286 The function of the human homolog of Saccharomyces cerevisiae REV1 is required for mutagenesis induced by UV light.

Gibbs, PE, Wang, XD, Li, Z, McManus, TP, McGregor, WG, Lawrence, CW, Maher, VM

Proc Natl Acad Sci U S A 2000
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