Reactome | REV1 binds AP-dsDNA

REV1 binds AP-dsDNA

Stable Identifier

R-HSA-110307

Type

Reaction [binding]

Species

Homo sapiens

Compartment

nucleoplasm

ReviewStatus

5/5

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REV1 is a deoxycytidyl transferase that belongs to the DNA polymerase type-Y family. REV1 was cloned as the human homolog of yeast REV1. Similar to its yeast counterpart, REV1 binds damaged DNA, with the preferred substrate being DNA with an AP (abasic - apurinic/apyrimidinic) site. The mechanism for DNA damage recognition has not been elucidated (Lin et al. 1999, Gibbs et al. 2000). Besides DNA binding, REV1 has a ubiquitin binding motif in its C-terminal domain that interacts with monoubiquitinated PCNA, which enhances REV1-mediated translesion synthesis (Garg and Burgers 2005, Wood et al. 2007).

Literature References

PubMed ID	Title	Journal	Year
17517887	A ubiquitin-binding motif in the translesion DNA polymerase Rev1 mediates its essential functional interaction with ubiquitinated proliferating cell nuclear antigen in response to DNA damage Wood, A, Garg, P, Burgers, PM	J. Biol. Chem.	2007
16344468	Ubiquitinated proliferating cell nuclear antigen activates translesion DNA polymerases eta and REV1 Garg, P, Burgers, PM	Proc. Natl. Acad. Sci. U.S.A.	2005
10536157	The human REV1 gene codes for a DNA template-dependent dCMP transferase. Lin, W, Xin, H, Zhang, Y, Wu, X, Yuan, F	Nucleic Acids Res	1999
10760286	The function of the human homolog of Saccharomyces cerevisiae REV1 is required for mutagenesis induced by UV light. Gibbs, PE, Wang, XD, Li, Z, McManus, TP, McGregor, WG, Lawrence, CW, Maher, VM	Proc Natl Acad Sci U S A	2000