L protein acts as a cap N7 methyltransferase to modify RSV mRNAs

Stable Identifier
R-HSA-9830848
Type
Reaction [omitted]
Species
Homo sapiens
Related Species
Human respiratory syncytial virus A
Compartment
cytosol
ReviewStatus
5/5
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L protein acts as a cap N7 methyltransferase to modify RSV mRNAs
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5' capping of respiratory syncytial virus (RSV) mRNAs is catalyzed by the polyribonucleotidyl transferase and methyltransferase domains of the catalytic subunit L of the RNA-dependent RNA polymerase (RdRP) complex. The capping of RSV mRNAs is a two-step process, involving cap0 and then cap1 creation. The so-called cap0, labeled m7GpppG- as all RSV transcripts start with guanidine (Liuzzi et al. 2005), is created through N7 methylation catalyzed by the methyltransferase domain of the L protein. The complex of L and P proteins is sufficient for the N7-methylation to occur. In RSV, N7-methylation (cap0 creation) precedes 2'-O-methylation (cap1 creation) (Sutto-Ortiz et al. 2021). 5' capping was confirmed in: 1C (NS1) mRNA (Collins and Wertz 1985; Liuzzi et al. 2005), 1B (NS2) mRNA (Collins and Wertz 1985; Liuzzi et al. 2005), N mRNA (Liuzzi et al. 2005), P mRNA (Liuzzi et al. 2005), M mRNA (Satake and Venkatesan 1984; Liuzzi et al. 2005), SH mRNA (Liuzzi et al. 2005), G mRNA (Liuzzi et al. 2005), F mRNA (Elango et al. 1985; Liuzzi et al. 2005), M2 mRNA (Liuzzi et al. 2005), and L mRNA (Liuzzi et al. 2005).
Literature References
PubMed ID Title Journal Year
2987829 Respiratory syncytial virus fusion glycoprotein: nucleotide sequence of mRNA, identification of cleavage activation site and amino acid sequence of N-terminus of F1 subunit

Coligan, JE, Elango, N, Venkatesan, S, Norrby, E, Satake, M, Camargo, E

Nucleic Acids Res 1985
6699948 Nucleotide sequence of the gene encoding respiratory syncytial virus matrix protein

Venkatesan, S, Satake, M

J Virol 1984
2998021 Nucleotide sequences of the 1B and 1C nonstructural protein mRNAs of human respiratory syncytial virus

Wertz, GW, Collins, PL

Virology 1985
33956914 The methyltransferase domain of the Respiratory Syncytial Virus L protein catalyzes cap N7 and 2'-O-methylation

Canard, B, Roymans, D, Sutto-Ortiz, P, Eléouët, JF, Decombe, A, Tcherniuk, S, Rigaux, P, Ysebaert, N, Decroly, E, Vasseur, JJ, Debart, F, Abeywickrema, P, Noël, M

PLoS Pathog 2021
16189012 Inhibitors of respiratory syncytial virus replication target cotranscriptional mRNA guanylylation by viral RNA-dependent RNA polymerase

Landry, S, Gaudette, Y, Lapeyre, N, Bordeleau, J, Massariol, MJ, Lamarre, L, Dô, F, Cartier, M, Simoneau, B, Dansereau, N, Scouten, E, McCollum, RS, Rancourt, J, Lagace, L, Mason, SW, Lawetz, C, Fazal, G, Liuzzi, M, Whitehead, P, Bolger, G

J Virol 2005
Participants
Input
Output
Participates
as an event of
Catalyst Activity

mRNA 5'-cap (guanine-N7-)-methyltransferase activity of hRSV A nascent mRNAs:RdRP:p-M2-1 [cytosol]

Physical Entity
hRSV A nascent mRNAs:RdRP:p-M2-1 [cytosol] (Human respiratory syncytial virus A)
Activity
mRNA 5'-cap (guanine-N7-)-methyltransferase activity (GO:0004482)
Disease
Authored
Orlic-Milacic, M  (2023-03-15)
Reviewed
Bergeron, HC  (2023-11-03)
Created
Orlic-Milacic, M  (2023-03-15)
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