The committed step of glycolysis is the phosphorylation of D-fructose 6-phosphate (Fru(6)P) to form D-fructose 1,6-bisphosphate, catalyzed by phosphofructokinase 1 (PFK) tetramer. PFK can be allosterically activated by D-fructose 2,6-bisphosphate whose levels are increased in response to insulin signaling and decreased in response to glucagon signaling, through the reactions annotated here (Pilkis et al. 1995).
