D-fructose 6-phosphate + ATP => D-fructose 2,6-bisphosphate + ADP

Stable Identifier
Reaction [transition]
Homo sapiens
Fructose 6-phosphate and ATP react to form fructose 2,6-bisphosphate and ADP
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The 6-phosphofructo-2-kinase activity of cytosolic PFKFB (6-phosphofructo-2-kinase/fructose-2,6-biphosphatase) homodimer catalyzes the reaction of fructose 6-phosphate and ATP to form fructose 2,6-bisphosphate and ADP (Pilkis et al. 1988). Fructose 2,6-bisphosphate is not itself on the pathway of glycolysis. Rather, it acts as a positive allosteric effector of phosphofructokinase 1, greatly increasing the rate of synthesis of fructose 1,6-bisphosphate and hence the overall rate of glycolysis. The conversion of PFKFB between its dephosphorylated form, which catalyzes the synthesis of fructose 2,6-bisphosphate as described here, and its phosphorylated form, which catalyzes the hydrolysis of fructose 2,6-bisphosphate to fructose 6-phosphate and orthophosphate plays a central role in the short-term regulation of glycolysis (Pilkis et al. 1995).

Four isoforms of PFKFB protein encoded by four different genes exhibit tissue-specific expression patterns. PFKFB1 is expressed in the liver (Algaier and Uyeda 1988), PFKFB2 is expressed in the heart (Hirata et al. 1998), PFKFB3 is ubiquitously expressed (Manes and el-Maghrabi 2005), and PFKFB4, originally described as a testis-specific gene product (Manzano et al. 1999), may also be expressed in several kinds of tumors.

Literature References
PubMed ID Title Journal Year
10095107 Cloning, expression and chromosomal localization of a human testis 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase gene

Manzano, A, Nadal, M, Lange, A, Perez, JX, Bartrons, R, Estivill, X

Gene 1999
15896703 The kinase activity of human brain 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase is regulated via inhibition by phosphoenolpyruvate

el-Maghrabi, MR, Manes, NP

Arch Biochem Biophys 2005
9464277 Expression of human placental-type 6-phosphofructo-2-kinase/fructose 2,6-bisphosphatase in various cells and cell lines

Okamura, N, Kato, M, Fukasawa, M, Sakakibara, R, Hirata, T

Biochem Biophys Res Commun 1998
3052289 Hormonal regulation of hepatic gluconeogenesis and glycolysis

el-Maghrabi, MR, Pilkis, SJ, Claus, TH

Annu Rev Biochem 1988
2837207 Molecular cloning, sequence analysis, and expression of a human liver cDNA coding for fructose-6-P,2-kinase:fructose-2,6-bisphosphatase

Uyeda, K, Algaier, J

Biochem Biophys Res Commun 1988
7574501 6-Phosphofructo-2-kinase/fructose-2,6-bisphosphatase: a metabolic signaling enzyme.

Kurland, IJ, Lange, AJ, Pilkis, SJ, Claus, TH

Annu Rev Biochem 1995
Event Information
Catalyst Activity

6-phosphofructo-2-kinase activity of PFKFB dimers [cytosol]

This event is regulated
Positively by
Orthologous Events
Cross References
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