Reactome | Removal of the Flap Intermediate

Removal of the Flap Intermediate

Stable Identifier

R-HSA-69166

Type

Pathway

Species

Homo sapiens

Compartment

nucleoplasm

ReviewStatus

5/5

Locations in the PathwayBrowser

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Cell Cycle (Homo sapiens)

- Cell Cycle, Mitotic (Homo sapiens)
  - S Phase (Homo sapiens)
    - Synthesis of DNA (Homo sapiens)
      - DNA strand elongation (Homo sapiens)
        
        Lagging Strand Synthesis (Homo sapiens)
        
        Processive synthesis on the lagging strand (Homo sapiens)
        
        Removal of the Flap Intermediate (Homo sapiens)

General

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Two endonucleases, Dna2 and flap endonuclease 1 (FEN-1), are responsible for resolving the nascent flap structure (Tsurimoto and Stillman 1991). The Dna2 endonuclease/helicase in yeast is a monomer of approximately 172 kDa. Human FEN-1 is a single polypeptide of approximately 42 kDa. Replication Protein A regulates the switching of endonucleases during the removal of the displaced flap (Tsurimoto et al.1991).

Literature References

PubMed ID	Title	Journal	Year
8131753	The characterization of a mammalian DNA structure-specific endonuclease. Harrington, JJ, Lieber, MR	EMBO J	1994
7644470	A yeast gene required for DNA replication encodes a protein with homology to DNA helicases. Campbell, JL, Budd, ME	Proc Natl Acad Sci U S A	1995
7926735	Functional domains within FEN-1 and RAD2 define a family of structure-specific endonucleases: implications for nucleotide excision repair. Harrington, JJ, Lieber, MR	Genes Dev	1994