Removal of remaining Flap

Stable Identifier
R-HSA-69152
Type
Reaction [transition]
Species
Homo sapiens
Compartment
nucleoplasm
ReviewStatus
5/5
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Removal of remaining Flap
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The remaining flap, which is too short to support RPA binding, is then processed by FEN-1. There is evidence that binding of RPA to the displaced end of the RNA-containing Okazaki fragment prevents FEN-1 from accessing the substrate. FEN-1 is a structure-specific endonuclease that cleaves near the base of the flap at a position one nucleotide into the annealed region. Biochemical studies have shown that the preferred substrate for FEN-1 consists of a one-nucleotide 3'-tail on the upstream primer in addition to the 5'-flap of the downstream primer.
Literature References
PubMed ID Title Journal Year
8131753 The characterization of a mammalian DNA structure-specific endonuclease.

Harrington, JJ, Lieber, MR

EMBO J 1994
7876218 DNA structural elements required for FEN-1 binding.

Harrington, JJ, Lieber, MR

J Biol Chem 1995
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Output
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Catalyst Activity

5'-flap endonuclease activity of FEN1 [nucleoplasm]

Physical Entity
FEN1 [nucleoplasm] (Homo sapiens)
Activity
5'-flap endonuclease activity (GO:0017108)
Orthologous Events
Removal of remaining Flap (Bos taurus)
Removal of remaining Flap (Caenorhabditis elegans)
Removal of remaining Flap (Canis familiaris)
Removal of remaining Flap (Dictyostelium discoideum)
Removal of remaining Flap (Drosophila melanogaster)
Removal of remaining Flap (Mus musculus)
Removal of remaining Flap (Plasmodium falciparum)
Removal of remaining Flap (Rattus norvegicus)
Removal of remaining Flap (Saccharomyces cerevisiae)
Removal of remaining Flap (Schizosaccharomyces pombe)
Removal of remaining Flap (Sus scrofa)
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