CCR4-NOT complex deadenylates mRNA

Stable Identifier
Reaction [transition]
Homo sapiens
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The CCR4-NOT complex hydrolyzes adenosine residues at the 3' end of polyadenylated mRNA, shortening the number of adenosine residues to about 10-15 residues and yielding adenosine 5'-monophosphate. CNOT6 and CNOT6L are the exoribonucleases responsible for hydrolysis. Activity of the CCR4-NOT complex is inhibited by PABP bound to the poly(A) tail of the mRNA. The trigger for activation of deadenylation by the CCR4-NOT complex is unknown. Complexes containing CNOT7 rather than CNOT8 appear to be responsible for cytoplasmic mRNA decay.
Literature References
PubMed ID Title Journal Year
14749774 The enzymes and control of eukaryotic mRNA turnover

Song, H, Parker, R

Nat Struct Mol Biol 2004
18625844 Deadenylation is prerequisite for P-body formation and mRNA decay in mammalian cells

Zhu, W, Ezzeddine, N, He, X, Zheng, D, Chen, CY, Shyu, AB

J Cell Biol 2008
16141059 From birth to death: the complex lives of eukaryotic mRNAs

Moore, MJ

Science 2005
15475613 Messenger RNA decay in mammalian cells: the exonuclease perspective

Fritz, DT, Wilusz, J, Bergman, N, Wilusz, CJ, Kilpatrick, WJ

Cell Biochem Biophys 2004
16284618 Concerted action of poly(A) nucleases and decapping enzyme in mammalian mRNA turnover

Zhu, W, Chang, TC, Yamashita, A, Zhong, Z, Yamashita, Y, Chen, CY, Shyu, AB

Nat Struct Mol Biol 2005
19239894 The many pathways of RNA degradation

Houseley, J, Tollervey, D

Cell 2009
19558367 Human Ccr4-Not complexes contain variable deadenylase subunits

Heck, AJ, van Schaik, FM, Lau, NC, Timmers, HT, Pijnappel, WW, Kolkman, A, Mulder, KW

Biochem J 2009
17245413 The highways and byways of mRNA decay

Garneau, NL, Wilusz, J, Wilusz, CJ

Nat Rev Mol Cell Biol 2007
10637334 Isolation and characterization of human orthologs of yeast CCR4-NOT complex subunits

Collart, MA, Lemaire, M, Timmers, HT, Gentz, R, Albert, TK, van Berkum, NL

Nucleic Acids Res 2000
11283721 The cap-to-tail guide to mRNA turnover

Wormington, M, Wilusz, CJ, Peltz, SW

Nat Rev Mol Cell Biol 2001
11889047 CCR4, a 3'-5' poly(A) RNA and ssDNA exonuclease, is the catalytic component of the cytoplasmic deadenylase

Chiang, YC, Denis, CL, Chen, J

EMBO J 2002
Catalyst Activity

poly(A)-specific ribonuclease activity of CCR4-NOT Complex [cytosol]

Orthologous Events
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