Reactome | CCR4-NOT complex deadenylates mRNA

CCR4-NOT complex deadenylates mRNA

Stable Identifier

R-HSA-429955

Type

Reaction [transition]

Species

Homo sapiens

Compartment

cytosol

ReviewStatus

5/5

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The CCR4-NOT complex hydrolyzes adenosine residues at the 3' end of polyadenylated mRNA, shortening the number of adenosine residues to about 10-15 residues and yielding adenosine 5'-monophosphate. CNOT6 and CNOT6L are the exoribonucleases responsible for hydrolysis. Activity of the CCR4-NOT complex is inhibited by PABP bound to the poly(A) tail of the mRNA. The trigger for activation of deadenylation by the CCR4-NOT complex is unknown. Complexes containing CNOT7 rather than CNOT8 appear to be responsible for cytoplasmic mRNA decay.

Literature References

PubMed ID	Title	Journal	Year
14749774	The enzymes and control of eukaryotic mRNA turnover Song, H, Parker, R	Nat Struct Mol Biol	2004
18625844	Deadenylation is prerequisite for P-body formation and mRNA decay in mammalian cells Zhu, W, Ezzeddine, N, He, X, Zheng, D, Chen, CY, Shyu, AB	J Cell Biol	2008
16141059	From birth to death: the complex lives of eukaryotic mRNAs Moore, MJ	Science	2005
15475613	Messenger RNA decay in mammalian cells: the exonuclease perspective Fritz, DT, Wilusz, J, Bergman, N, Wilusz, CJ, Kilpatrick, WJ	Cell Biochem Biophys	2004
16284618	Concerted action of poly(A) nucleases and decapping enzyme in mammalian mRNA turnover Zhu, W, Chang, TC, Yamashita, A, Zhong, Z, Yamashita, Y, Chen, CY, Shyu, AB	Nat Struct Mol Biol	2005
19239894	The many pathways of RNA degradation Houseley, J, Tollervey, D	Cell	2009
19558367	Human Ccr4-Not complexes contain variable deadenylase subunits Heck, AJ, van Schaik, FM, Lau, NC, Timmers, HT, Pijnappel, WW, Kolkman, A, Mulder, KW	Biochem J	2009
17245413	The highways and byways of mRNA decay Garneau, NL, Wilusz, J, Wilusz, CJ	Nat Rev Mol Cell Biol	2007
10637334	Isolation and characterization of human orthologs of yeast CCR4-NOT complex subunits Collart, MA, Lemaire, M, Timmers, HT, Gentz, R, Albert, TK, van Berkum, NL	Nucleic Acids Res	2000
11283721	The cap-to-tail guide to mRNA turnover Wormington, M, Wilusz, CJ, Peltz, SW	Nat Rev Mol Cell Biol	2001
11889047	CCR4, a 3'-5' poly(A) RNA and ssDNA exonuclease, is the catalytic component of the cytoplasmic deadenylase Chiang, YC, Denis, CL, Chen, J	EMBO J	2002