Reactome | NS5 synthesizes minus strand genome

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NS5 synthesizes minus strand genome Show undefined attributes

authored	[InstanceEdit:9922933] Stephan, Ralf, 2024-09-27
catalystActivity	[CatalystActivity:9922962] RNA-directed RNA polymerase activity of DENV circular genomic +ssRNA:RC [endoplasmic reticulum membrane]
category	uncertain
compartment	[Compartment:70101] cytosol
created	[InstanceEdit:9922958] Stephan, Ralf, 2024-09-28
crossReference	[DatabaseIdentifier:12020825] Mondo:0005502 [DatabaseIdentifier:9923115] RHEA:21249
dbId	9922955
disease	[Disease:3244610] dengue disease
displayName	NS5 synthesizes minus strand genome
edited	[InstanceEdit:9966335] Stephan, Ralf, 2025-09-18
eventOf	[Pathway:R-HSA-9918487] Dengue Virus Genome Translation and Replication - Homo sapiens
followingEvent	[BlackBoxEvent:R-HSA-9923073] NS3 unwinds dsRNA secondary structures - Homo sapiens [Reaction:R-HSA-9947672] YBX1 binds DENV mRNA - Homo sapiens [Reaction:R-HSA-9948218] HDLBP and RRBP1 bind DENV mRNA - Homo sapiens [BlackBoxEvent:R-HSA-9947293] IRES-mediated translation of DENV genomic RNA - Homo sapiens [Reaction:R-HSA-9922961] DENV genomic RNA binds Replication Complex - Homo sapiens
goBiologicalProcess	[GO_BiologicalProcess:5083347] viral RNA genome replication
input	[Complex:R-DVI-9979976] DENV circular genomic +ssRNA:RC [endoplasmic reticulum membrane] [SimpleEntity:R-ALL-6806881] NTP [cytosol]
isChimeric	false
isInDisease	true
isInferred	false
literatureReference	[LiteratureReference:9919995] Molecular Insights into the Flavivirus Replication Complex [LiteratureReference:9925592] Recombinant dengue type 1 virus NS5 protein expressed in Escherichia coli exhibits RNA-dependent RNA polymerase activity [LiteratureReference:9946465] A conserved arginine in NS5 binds genomic 3' stem-loop RNA for primer-independent initiation of flavivirus RNA replication [LiteratureReference:9946499] The Role of the Stem-Loop A RNA Promoter in Flavivirus Replication [LiteratureReference:9956275] Identification of a Broad-Spectrum Viral Inhibitor Targeting a Novel Allosteric Site in the RNA-Dependent RNA Polymerases of Dengue Virus and Norovirus [LiteratureReference:9956248] Efficacy of the broad-spectrum antiviral compound BCX4430 against Zika virus in cell culture and in a mouse model [LiteratureReference:9956278] Targeting the RdRp of Emerging RNA Viruses: The Structure-Based Drug Design Challenge [LiteratureReference:9956251] Evaluation of Sofosbuvir (?-D-2'-deoxy-2'-?-fluoro-2'-?-C-methyluridine) as an inhibitor of Dengue virus replication<sup/> [LiteratureReference:9956255] The clinically approved antiviral drug sofosbuvir inhibits Zika virus replication
maxDepth	1
modified	[InstanceEdit:9984898] Weiser, Joel, 2026-03-12
name	NS5 synthesizes minus strand genome
output	[Complex:R-DVI-9979951] DENV -ssRNA genome replicate with secondary dsRNA structure:RC [endoplasmic reticulum membrane] [EntityWithAccessionedSequence:R-DVI-9922609] DENV m7G(5')pppAm-capped genomic +ssRNA [cytosol] [SimpleEntity:R-ALL-111294] PPi [cytosol]
precedingEvent	[BlackBoxEvent:R-HSA-9922965] Circularization of (+)-genomic RNA - Homo sapiens
previousReviewStatus	[ReviewStatus:9821384] two stars
regulatedBy	[NegativeRegulation:9955889] Negative regulation by 'DENV polymerase inhibitors [cytosol]'
relatedSpecies	[Species:3244621] Dengue virus type 2
releaseDate	2025-12-08
releaseStatus	UPDATED
reviewStatus	[ReviewStatus:9821382] five stars
reviewed	[InstanceEdit:9966332] Mansur, Daniel Santos, 2025-09-18 [InstanceEdit:9970439] Roque Ascen��o, Fernando, 2025-10-29 [InstanceEdit:9984049] Roque Ascen��o, Fernando, 2026-03-03
schemaClass	BlackBoxEvent
species	[Species:48887] Homo sapiens
stId	R-HSA-9922955
summation	[Summation:9923693] RNA-dependent RNA polymerase (RdRp) domain of NS5 subunit of...
updateTrackers	[UpdateTracker:9984720] Update Tracker - [BlackBoxEvent:9922955] NS5 synthesizes minus strand genome - v96:[add_removeInput, add_removeOutput]

Referrals

(updatedInstance)	[UpdateTracker:9984720] Update Tracker - [BlackBoxEvent:9922955] NS5 synthesizes minus strand genome - v96:[add_removeInput, add_removeOutput]
(hasEvent)	[Pathway:R-HSA-9918487] Dengue Virus Genome Translation and Replication
(precedingEvent)	[Reaction:R-HSA-9947672] YBX1 binds DENV mRNA [Reaction:R-HSA-9948218] HDLBP and RRBP1 bind DENV mRNA [BlackBoxEvent:R-HSA-9947293] IRES-mediated translation of DENV genomic RNA [NegativePrecedingEvent:9960011] Negative relationship: NS5 synthesizes minus strand genome [NegativePrecedingEvent:9960008] Negative relationship: NS5 synthesizes minus strand genome [BlackBoxEvent:R-HSA-9923073] NS3 unwinds dsRNA secondary structures [Reaction:R-HSA-9922961] DENV genomic RNA binds Replication Complex

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