Reactome | nsp14 acts as a 3'-to-5' exonuclease to remove misincorporated nucleotides from nascent RNA

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nsp14 acts as a 3'-to-5' exonuclease to remove misincorporated nucleotides from nascent RNA Show undefined attributes

authored	[InstanceEdit:9697308] Orlic-Milacic, Marija, 2020-08-12
catalystActivity	[CatalystActivity:9694426] 3'-5'-RNA exonuclease activity of SARS coronavirus gRNA:RTC:nascent RNA minus strand with mismatched nucleotide [double membrane vesicle viral factory outer membrane]
category	transition
compartment	[Compartment:70101] cytosol [Compartment:9684675] double membrane vesicle viral factory outer membrane
created	[InstanceEdit:9694660] Cook, Justin, 2020-07-07
crossReference	[DatabaseIdentifier:12020746] Mondo:0100096
dbId	9694632
disease	[Disease:9683912] COVID-19
displayName	nsp14 acts as a 3'-to-5' exonuclease to remove misincorporated nucleotides from nascent RNA
edited	[InstanceEdit:9697657] Orlic-Milacic, Marija, 2020-08-18
eventOf	[Pathway:R-HSA-9694686] Replication of the SARS-CoV-2 genome - Homo sapiens
followingEvent	[BlackBoxEvent:R-HSA-9694549] RTC completes synthesis of the minus strand genomic RNA complement - Homo sapiens [Reaction:R-HSA-9694665] SARS-CoV-2 gRNA:RTC:nascent RNA minus strand binds RTC inhibitors - Homo sapiens
input	[SimpleEntity:R-ALL-29356] H2O [cytosol] [Complex:R-COV-9694269] SARS coronavirus gRNA:RTC:nascent RNA minus strand with mismatched nucleotide [double membrane vesicle viral factory outer membrane]
isChimeric	false
isInDisease	true
isInferred	false
literatureReference	[LiteratureReference:9713216] The Enzymatic Activity of the nsp14 Exoribonuclease Is Critical for Replication of MERS-CoV and SARS-CoV-2 [LiteratureReference:9763853] Mutations of SARS-CoV-2 nsp14 exhibit strong association with increased genome-wide mutation load [URL:9763827] https://doi.org/10.1101/2020.12.23.424231 [LiteratureReference:9763829] Structural basis of mismatch recognition by a SARS-CoV-2 proofreading enzyme [LiteratureReference:9763840] Reconstitution and functional characterization of SARS-CoV-2 proofreading complex [LiteratureReference:9763844] Structure and dynamics of SARS-CoV-2 proofreading exoribonuclease ExoN [LiteratureReference:9762576] New targets for drug design: importance of nsp14/nsp10 complex formation for the 3'-5' exoribonucleolytic activity on SARS-CoV-2 [LiteratureReference:9763858] Activation of the SARS-CoV-2 NSP14 3'-5' exoribonuclease by NSP10 and response to antiviral inhibitors [LiteratureReference:9756106] Crystal structure of SARS-CoV-2 nsp10 bound to nsp14-ExoN domain reveals an exoribonuclease with both structural and functional integrity
maxDepth	1
modified	[InstanceEdit:9830342] Matthews, Lisa, 2023-03-08
name	nsp14 acts as a 3'-to-5' exonuclease to remove misincorporated nucleotides from nascent RNA
output	[SimpleEntity:R-ALL-9689912] NMP [cytosol] [Complex:R-COV-9694618] SARS-CoV-2 gRNA:RTC:nascent RNA minus strand [double membrane vesicle viral factory outer membrane]
precedingEvent	[Reaction:R-HSA-9815529] nsp12 transfers guanylyl onto SARS-CoV-2 plus strand subgenomic RNAs - Homo sapiens
relatedSpecies	[Species:9681683] Severe acute respiratory syndrome coronavirus 2
releaseDate	2020-09-15
reviewStatus	[ReviewStatus:9821382] five stars
reviewed	[InstanceEdit:9699044] Acencio, Marcio Luis, 2020-09-09
schemaClass	Reaction
species	[Species:48887] Homo sapiens
stId	R-HSA-9694632
summation	[Summation:9694564] In human SARS coronavirus 2 (SARS-CoV-2) nsp14 is essential ...
updateTrackers	[UpdateTracker:9776447] Update Tracker - [Reaction:9694632] nsp14 acts as a 3'-to-5' exonuclease to remove misincorporated nucleotides from nascent RNA - v76:[addLiteratureReference, modifyText] [UpdateTracker:9774952] Update Tracker - [Reaction:9694632] nsp14 acts as a 3'-to-5' exonuclease to remove misincorporated nucleotides from nascent RNA - v80:[addLiteratureReference, modifyText]

Referrals

(updatedInstance)	[UpdateTracker:9776447] Update Tracker - [Reaction:9694632] nsp14 acts as a 3'-to-5' exonuclease to remove misincorporated nucleotides from nascent RNA - v76:[addLiteratureReference, modifyText] [UpdateTracker:9774952] Update Tracker - [Reaction:9694632] nsp14 acts as a 3'-to-5' exonuclease to remove misincorporated nucleotides from nascent RNA - v80:[addLiteratureReference, modifyText]
(hasEvent)	[Pathway:R-HSA-9694686] Replication of the SARS-CoV-2 genome
(precedingEvent)	[NegativePrecedingEvent:9763943] Futile Cycle: nsp14 acts as a 3'-to-5' exonuclease to remove misincorporated nucleotides from nascent RNA [Reaction:R-HSA-9694665] SARS-CoV-2 gRNA:RTC:nascent RNA minus strand binds RTC inhibitors [BlackBoxEvent:R-HSA-9694549] RTC completes synthesis of the minus strand genomic RNA complement

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