Ferriheme dissociates from p-T-Eif2ak1:2xferriheme dimer

Stable Identifier
R-MMU-9655598
Type
Reaction [dissociation]
Species
Mus musculus
Compartment
General
SVG |   | PPTX  | SBGN
Ferriheme dissociates from p-T-Eif2ak1:2xferriheme dimer

One molecule of hemin (ferriheme b chloride) tightly binds the N-terminal domain of Eif2ak1 (HRI) and one molecule of hemin loosely binds the kinase insert (KI) domain of Eif2ak1 (Misanova et al. 2006, Hirai et al. 2007, Igarashi et al. 2008). When cytosolic heme concentrations are low, heme dissociates from the KI domain, resulting in activation of the kinase activity of Eif2ak1 (Miksanova et al. 2006, Hirai et al. 2007, Igarashi et al. 2008).

Literature References
PubMed ID Title Journal Year
18450746 Elucidation of the heme binding site of heme-regulated eukaryotic initiation factor 2alpha kinase and the role of the regulatory motif in heme sensing by spectroscopic and catalytic studies of mutant proteins

Igarashi, J, Murase, M, Iizuka, A, Pichierri, F, Martinkova, M, Shimizu, T

J. Biol. Chem. 2008
17597215 Identification of Cys385 in the isolated kinase insertion domain of heme-regulated eIF2 alpha kinase (HRI) as the heme axial ligand by site-directed mutagenesis and spectral characterization

Hirai, K, Martinkova, M, Igarashi, J, Saiful, I, Yamauchi, S, El-Mashtoly, S, Kitagawa, T, Shimizu, T

J. Inorg. Biochem. 2007
16893190 Characterization of heme-regulated eIF2alpha kinase: roles of the N-terminal domain in the oligomeric state, heme binding, catalysis, and inhibition

Miksanova, M, Igarashi, J, Minami, M, Sagami, I, Yamauchi, S, Kurokawa, H, Shimizu, T

Biochemistry 2006
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