RTC completes synthesis of the minus strand genomic RNA complement

Stable Identifier
R-HSA-9682465
Type
Reaction [uncertain]
Species
Homo sapiens
Related Species
Human SARS coronavirus
Compartment
cytosol, double membrane vesicle viral factory outer membrane
ReviewStatus
5/5
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RTC completes synthesis of the minus strand genomic RNA complement
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The replication-transcription complex (RTC) completes synthesis of the genomic RNA complement (minus strand). The complex of nsp7 and nsp8 confers processivity to nsp12, the virally encoded RNA-dependent RNA polymerase that replicates the viral genomic RNA, enabling the RTC to complete the RNA synthesis with a very low dissociation rate. nsp7 plays a crucial role in maintaining binding of the RTC to the RNA. nsp14 subunit of the RTC does not affect the processivity (Subissi et al. 2014).
Literature References
PubMed ID Title Journal Year
25197083 One severe acute respiratory syndrome coronavirus protein complex integrates processive RNA polymerase and exonuclease activities

Canard, B, Collet, A, Gorbalenya, AE, Subissi, L, Decroly, E, Imbert, I, Posthuma, CC, Zevenhoven-Dobbe, JC, Snijder, EJ

Proc. Natl. Acad. Sci. U.S.A. 2014
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Output
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as an event of
Catalyst Activity

RNA-directed 5'-3' RNA polymerase activity of SARS-CoV-1 gRNA:RTC:nascent RNA minus strand [double membrane vesicle viral factory outer membrane]

Physical Entity
SARS-CoV-1 gRNA:RTC:nascent RNA minus strand [double membrane vesicle viral factory outer membrane] (Human SARS coronavirus)
Activity
RNA-directed 5'-3' RNA polymerase activity (GO:0003968)
This event is regulated
Negatively by
Regulator
SARS-CoV-1 gRNA:RTC:nascent RNA minus strand:RTC inhibitors [double membrane vesicle viral factory outer membrane] (Human SARS coronavirus)
Orthologous Events
RTC completes synthesis of the minus strand genomic RNA complement (Homo sapiens)
Disease
Name Identifier Synonyms
severe acute respiratory syndrome DOID:2945 SARS-CoV infection, SARS
Authored
Orlic-Milacic, M  (2020-04-30)
Reviewed
Acencio, ML  (2020-05-27)
Mazein, A  (2020-05-27)
Created
Orlic-Milacic, M  (2020-04-08)
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