RTC completes synthesis of the minus strand genomic RNA complement

Stable Identifier
R-HSA-9694549
Type
Reaction [uncertain]
Species
Homo sapiens
Related Species
Severe acute respiratory syndrome coronavirus 2
Compartment
cytosol, double membrane vesicle viral factory outer membrane
ReviewStatus
5/5
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RTC completes synthesis of the minus strand genomic RNA complement
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This COVID-19 event has been created by a combination of computational inference (see https://reactome.org/documentation/inferred-events) from SARS-CoV-1 data and manual curation, as described in the summation for the overall SARS-CoV-2 infection pathway.

The replication-transcription complex (RTC) completes synthesis of the genomic RNA complement (minus strand). The complex of nsp7 and nsp8 confers processivity to nsp12, the virally encoded RNA-dependent RNA polymerase that replicates the viral genomic RNA, enabling the RTC to complete the RNA synthesis with a very low dissociation rate. nsp7 plays a crucial role in maintaining binding of the RTC to the RNA. nsp14 subunit of the RTC does not affect the processivity (Subissi et al. 2014).
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Output
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as an event of
Catalyst Activity

RNA-dependent RNA polymerase activity of SARS-CoV-2 gRNA:RTC:nascent RNA minus strand [double membrane vesicle viral factory outer membrane]

Physical Entity
SARS-CoV-2 gRNA:RTC:nascent RNA minus strand [double membrane vesicle viral factory outer membrane] (Severe acute respiratory syndrome coronavirus 2)
Activity
RNA-dependent RNA polymerase activity (GO:0003968)
This event is regulated
Negatively by
Regulator
SARS-CoV-2 gRNA:RTC:nascent RNA minus strand:RTC inhibitors [double membrane vesicle viral factory outer membrane] (Severe acute respiratory syndrome coronavirus 2)
Inferred From
RTC completes synthesis of the minus strand genomic RNA complement (Homo sapiens)
Disease
Name Identifier Synonyms
COVID-19 DOID:0080600 2019 Novel Coronavirus (2019-nCoV), Wuhan seafood market pneumonia virus infection, 2019-nCoV infection, Wuhan coronavirus infection
Authored
Orlic-Milacic, M  (2020-08-12)
Reviewed
Acencio, ML  (2020-09-09)
Created
Cook, J  (2020-07-07)
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