p-SHC1 binds integrin alpha5beta1:fibronectin

Stable Identifier
Reaction [binding]
Homo sapiens
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Estradiol treatment of human breast cancer cells stimulates GPER1-dependent formation of integrin alpha5 beta1:fibronectin (FN):phosphorylated SHC1 complexes in a SRC- and G-protein beta gamma-dependent fashion (Quinn et al, 2009). Formation of integrin:fibronectin fibrils has been shown to enhance anchorage independent growth (Salnier et al, 1996; Qiao et al, 2000; Quinn et al, 2009). Formation of a fibronectin:integrin matrix is required for estradiol-stimulated EGFR phosphorylation, and consistent with this, EGFR phosphorylation is inhibited after treatment of cells with either soluble FN peptide fragments or antibody that blocks the integrin alpha5beta1:fibronectin interaction (Quinn et al, 2009; reviewed in Prossnitz and Barton, 2014). Although SHC is known to have roles downstream of activated EGFR, EGFR phosphorylation after estradiol stimulation is abrogated in the inactive SHC Y317F mutant, suggesting that SHC acts upstream of EGFR in this pathway. In contrast, SHC phosphorylation is not required for the interaction with integrin alpha5beta1 (Quinn et al, 2009).

Literature References
PubMed ID Title Journal Year
8598224 Fibronectin fibrils and growth factors stimulate anchorage-independent growth of a murine mammary carcinoma

Mosher, D, Klassen, J, Bhardwaj, B, Leopold, D, Saulnier, R, Rahimi, N, Elliott, B, Tremblay, E

Exp. Cell Res. 1996
19342448 Coordinate regulation of estrogen-mediated fibronectin matrix assembly and epidermal growth factor receptor transactivation by the G protein-coupled receptor, GPR30

Schwarzbauer, JE, Frackelton, AR, Kim, M, Filardo, EJ, Graeber, CT, Quinn, JA

Mol. Endocrinol. 2009
21844907 The G-protein-coupled estrogen receptor GPER in health and disease

Prossnitz, ER, Barton, M

Nat Rev Endocrinol 2011
10714768 Cooperative effect of hepatocyte growth factor and fibronectin in anchorage-independent survival of mammary carcinoma cells: requirement for phosphatidylinositol 3-kinase activity

Qiao, H, Patryzkat, A, Rossiter, J, Raptis, L, Rahimi, N, Elliott, B, Tremblay, E, Saulnier, R

Cell Growth Differ. 2000
Orthologous Events
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