Reactome | TREX complex binds spliced, capped mRNA:CBC:EJC cotranscriptionally

TREX complex binds spliced, capped mRNA:CBC:EJC cotranscriptionally

Stable Identifier

R-HSA-8849157

Type

Reaction [binding]

Species

Homo sapiens

Compartment

nucleoplasm

ReviewStatus

5/5

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TREX complex binds spliced, capped mRNA:CBC:EJC cotranscriptionally

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The THO subcomplex of the TREX complex initially interacts with the serine-2,5 phosphorylated C-terminal domain of RNA polymerase II (Strasser et al. 2002, Inferred from yeast in Meinel et al. 2013) then with CBP80 of the cap binding complex (Cheng et al. 2006, Dufu et al. 2010, Chi et al. 2013). A TREX complex binds spliced mRNA near the cap during transcription (Cheng et al. 2006). Recruitment is dependent on splicing of the mRNA (Masuda et al. 2005). THO/TREX is required for efficient mRNP biogenesis and export (reviewed in Luna et al 2012). In yeast, components of the THO/TREX complex also affect transcription and 3' processing of mRNA, (Rondon et al. 2003, Rougemaille et al. 2008, Johnson et al. 2011, reviewed in Katahira 2015), however the human TREX complex does not appear to affect transcription (Masuda et al. 2005). The AREX complex, which contains DDX39A (UHR49) rather than DDX39B (UAP56) appears to perform the same function as TREX in mRNA export, but acts on a different subset of mRNAs (Yamazaki et al. 2010).

Literature References

PubMed ID	Title	Journal	Year
21947206	The export factor Yra1 modulates mRNA 3' end processing Johnson, SA, Kim, H, Erickson, B, Bentley, DL	Nat. Struct. Mol. Biol.	2011
18957205	THO/Sub2p functions to coordinate 3'-end processing with gene-nuclear pore association Rougemaille, M, Dieppois, G, Kisseleva-Romanova, E, Gudipati, RK, Lemoine, S, Blugeon, C, Boulay, J, Jensen, TH, Stutz, F, Devaux, F, Libri, D	Cell	2008
15998806	Recruitment of the human TREX complex to mRNA during splicing Masuda, S, Das, R, Cheng, H, Hurt, E, Dorman, N, Reed, R	Genes Dev.	2005
12871933	Molecular evidence that the eukaryotic THO/TREX complex is required for efficient transcription elongation Rondón, AG, Jimeno, S, García-Rubio, M, Aguilera, A	J. Biol. Chem.	2003
22207203	New clues to understand the role of THO and other functionally related factors in mRNP biogenesis Luna, R, Rondón, AG, Aguilera, A	Biochim. Biophys. Acta	2012
22928037	The proteins PDIP3 and ZC11A associate with the human TREX complex in an ATP-dependent manner and function in mRNA export Folco, EG, Lee, CS, Dufu, K, Yamazaki, T, Reed, R	PLoS ONE	2012
23299939	Chtop is a component of the dynamic TREX mRNA export complex Chang, CT, Hautbergue, GM, Walsh, MJ, Viphakone, N, van Dijk, TB, Philipsen, S, Wilson, SA	EMBO J.	2013
23222130	Aly and THO are required for assembly of the human TREX complex and association of TREX components with the spliced mRNA Chi, B, Wang, Q, Wu, G, Tan, M, Wang, L, Shi, M, Chang, X, Cheng, H	Nucleic Acids Res.	2013
25836925	Nuclear export of messenger RNA Katahira, J	Genes (Basel)	2015
19836239	UIF, a New mRNA export adaptor that works together with REF/ALY, requires FACT for recruitment to mRNA Hautbergue, GM, Hung, ML, Walsh, MJ, Snijders, AP, Chang, CT, Jones, R, Ponting, CP, Dickman, MJ, Wilson, SA	Curr. Biol.	2009
17190602	Human mRNA export machinery recruited to the 5' end of mRNA Cheng, H, Dufu, K, Lee, CS, Hsu, JL, Dias, A, Reed, R	Cell	2006
20844015	ATP is required for interactions between UAP56 and two conserved mRNA export proteins, Aly and CIP29, to assemble the TREX complex Dufu, K, Livingstone, MJ, Seebacher, J, Gygi, SP, Wilson, SA, Reed, R	Genes Dev.	2010
25662211	Luzp4 defines a new mRNA export pathway in cancer cells Viphakone, N, Cumberbatch, MG, Livingstone, MJ, Heath, PR, Dickman, MJ, Catto, JW, Wilson, SA	Nucleic Acids Res.	2015
24244187	Recruitment of TREX to the transcription machinery by its direct binding to the phospho-CTD of RNA polymerase II Meinel, DM, Burkert-Kautzsch, C, Kieser, A, O'Duibhir, E, Siebert, M, Mayer, A, Cramer, P, Söding, J, Holstege, FC, Strässer, K	PLoS Genet.	2013
20573985	The closely related RNA helicases, UAP56 and URH49, preferentially form distinct mRNA export machineries and coordinately regulate mitotic progression Yamazaki, T, Fujiwara, N, Yukinaga, H, Ebisuya, M, Shiki, T, Kurihara, T, Kioka, N, Kambe, T, Nagao, M, Nishida, E, Masuda, S	Mol. Biol. Cell	2010
17984224	ATP-dependent recruitment of export factor Aly/REF onto intronless mRNAs by RNA helicase UAP56 Taniguchi, I, Ohno, M	Mol. Cell. Biol.	2008
18411249	Binding of ATP to UAP56 is necessary for mRNA export Kota, KP, Wagner, SR, Huerta, E, Underwood, JM, Nickerson, JA	J. Cell. Sci.	2008
11979277	TREX is a conserved complex coupling transcription with messenger RNA export Strässer, K, Masuda, S, Mason, P, Pfannstiel, J, Oppizzi, M, Rodriguez-Navarro, S, Rondón, AG, Aguilera, A, Struhl, K, Reed, R, Hurt, E	Nature	2002