Cytosolic purine 5'-nucleotidase (NT5C2) catalyzes the hydrolysis of GMP, dGMP, IMP, and dIMP to yield the corresponding nucleosides and orthophosphate (Bianchi and Spychala 2003; Spychala et al. 1988). The active form of the enzyme is a tetramer and has an absolute requirement for magnesium ions (Spychala et al. 1988; Wallden et al. 2007). Consistent with the biochemical properties of purified enzyme, cultured cells overexpressing the enzyme from a recombinant DNA clone showed enhanced activity against inosine and guanosine monophosphates, but not adenosine monophosphate (Gazziola et al. 2001; Sala-Newby et al. 2000).
Sala-Newby, GB, Freeman, NV, Skladanowski, A, Newby, AC
Bianchi, V, Spychala, J
Gazziola, C, Ferraro, P, Moras, M, Reichard, PA, Bianchi, V
Spychala, J, Madrid-Marina, V, Fox, IH
Wallden, K, Stenmark, P, Nyman, T, Flodin, S, Graslund, S, Loppnau, P, Bianchi, V, Nordlund, P
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