Reactome | (d)GMP or (d)IMP + H2O => (2'-deoxy)guanosine or (2'-deoxy)inosine + orthophosphate (NT5C2)

(d)GMP or (d)IMP + H2O => (2'-deoxy)guanosine or (2'-deoxy)inosine + orthophosphate (NT5C2)

Stable Identifier

R-HSA-74248

Type

Reaction [transition]

Species

Homo sapiens

Compartment

cytosol

ReviewStatus

5/5

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(d)GMP or (d)IMP + H2O => (2'-deoxy)guanosine or (2'-deoxy)inosine + orthophosphate (NT5C2)

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Cytosolic purine 5'-nucleotidase (NT5C2) catalyzes the hydrolysis of GMP, dGMP, IMP, and dIMP to yield the corresponding nucleosides and orthophosphate (Bianchi and Spychala 2003; Spychala et al. 1988). The active form of the enzyme is a tetramer and has an absolute requirement for magnesium ions (Spychala et al. 1988; Wallden et al. 2007). Consistent with the biochemical properties of purified enzyme, cultured cells overexpressing the enzyme from a recombinant DNA clone showed enhanced activity against inosine and guanosine monophosphates, but not adenosine monophosphate (Gazziola et al. 2001; Sala-Newby et al. 2000).

Literature References

PubMed ID	Title	Journal	Year
12947102	Mammalian 5'-nucleotidases Spychala, J, Bianchi, V	J Biol Chem	2003
10766785	Distinct roles for recombinant cytosolic 5'-nucleotidase I and -II in AMP and IMP catabolism in COS-7 and H9c2 rat myoblast cell lines Skladanowski, A, Newby, AC, Sala-Newby, GB, Freeman, NV	J Biol Chem	2000
11083867	Cytosolic high Km 5'-nucleotidase and 5'(3')-deoxyribonucleotidase in substrate cycles involved in nucleotide metabolism Bianchi, V, Moras, M, Reichard, PA, Ferraro, P, Gazziola, C	J Biol Chem	2001
2848805	High Km soluble 5'-nucleotidase from human placenta Spychala, J, Fox, IH, Madrid-Marina, V	J Biol Chem	1988
17405878	Crystal structure of human cytosolic 5'-nucleotidase II: insights into allosteric regulation and substrate recognition Bianchi, V, Nordlund, P, Stenmark, P, Flodin, S, Nyman, T, Wallden, K, Loppnau, P, Graslund, S	J Biol Chem	2007