WHSC1 dimethylates histone H4 on lysine K21 at DSBs

Stable Identifier
R-HSA-5682965
Type
Reaction [transition]
Species
Homo sapiens
Compartment
nucleoplasm
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WHSC1 dimethylates histone H4 on lysine K21 at DSBs
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WHSC1 (MMSET) histone methyltransferase dimethylates histone H4 (HIST1H4A) on lysine residue K21 (commonly labeled in literature as K20), locally increasing the concentration of the H4K20Me2 mark. H4K20Me2 (Me2-K21-HIST1H4A) serves as a binding site for TP53BP1 (53BP1). The recruitment of WHSC1 to DNA double strand breaks (DSBs) is independent of RNF8 and RNF168, but the catalytic activity of all three proteins is necessary for binding and accumulation of TP53BP1 at DSBs (Pei et al. 2011).

Literature References
PubMed ID Title Journal Year
21293379 MMSET regulates histone H4K20 methylation and 53BP1 accumulation at DNA damage sites

Pei, H, Zhang, L, Luo, K, Qin, Y, Chesi, M, Fei, F, Bergsagel, PL, Wang, L, You, Z, Lou, Z

Nature 2011
Participants
Input
Output
Participant Of
hasEvent
Catalyst Activity
Catalyst Activity
Title
histone methyltransferase activity (H4-K20 specific) of DNA DSBs:p-MRN:p-S1981,Ac-K3016-ATM:KAT5:p-S139-H2AFX-Nucleosome:p-5T-MDC1:p-S102-WHSC1 [nucleoplasm]
Physical Entity
DNA DSBs:p-MRN:p-S1981,Ac-K3016-ATM:KAT5:p-S139-H2AFX-Nucleosome:p-5T-MDC1:p-S102-WHSC1 [nucleoplasm]
Activity
histone methyltransferase activity (H4-K20 specific) (0042799)
Orthologous Events
WHSC1 dimethylates histone H4 on lysine K21 at DSBs (Bos taurus)
WHSC1 dimethylates histone H4 on lysine K21 at DSBs (Caenorhabditis elegans)
WHSC1 dimethylates histone H4 on lysine K21 at DSBs (Canis familiaris)
WHSC1 dimethylates histone H4 on lysine K21 at DSBs (Danio rerio)
WHSC1 dimethylates histone H4 on lysine K21 at DSBs (Drosophila melanogaster)
WHSC1 dimethylates histone H4 on lysine K21 at DSBs (Gallus gallus)
WHSC1 dimethylates histone H4 on lysine K21 at DSBs (Mus musculus)
WHSC1 dimethylates histone H4 on lysine K21 at DSBs (Rattus norvegicus)
WHSC1 dimethylates histone H4 on lysine K21 at DSBs (Schizosaccharomyces pombe)
WHSC1 dimethylates histone H4 on lysine K21 at DSBs (Sus scrofa)
Authored
Orlic-Milacic, M  (2015-05-12)
Reviewed
Borowiec, JA  (2015-06-12)
Created
Orlic-Milacic, M  (2015-03-11)