Translesion synthesis by POLK

Stable Identifier
Homo sapiens
Translesion Synthesis by DNA Polymerase Kappa
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DNA polymerase kappa (POLK) is a Y family DNA polymerase that is most efficient in translesion DNA synthesis (TLS) across oxidation derivatives of DNA bases, such as thymine glycol (Tg) and 8-oxoguanine (OGUA), as well as bulky DNA adducts, such as benzo(a)pyrene diol epoxide guanine adduct (BPDE-G) (Zhang et al. 2000, Fischhaber et al.2002, Avkin et al. 2004, Vasquez-Del Carpio et al. 2009, Yoon et al. 2010, Lior-Hoffmann et al. 2012, Christov et al. 2012, Yoon et al. 2014). POLK carries out TLS by forming a quaternary complex with REV1 and POLZ (REV3L:MAD2L2) at DNA damage sites, where POLK simultaneously binds REV1 and monoubiquitinated PCNA (Ohashi et al. 2009, Haracska, Unk et al. 2002, Bi et al. 2006). POLK and POLZ cooperate in the elongation of nucleotides inserted opposite to lesioned bases by POLK. Similarly to POLZ, POLK has low processivity and is error-prone (Ohashi et al. 2000, Haracska, Prakash et al. 2002, Yoon et al. 2010).
Literature References
PubMed ID Title Journal Year
24648516 A role for DNA polymerase ? in promoting replication through oxidative DNA lesion, thymine glycol, in human cells

Prakash, L, Roy Choudhury, J, Yoon, JH, Park, J, Prakash, S

J. Biol. Chem. 2014
11058110 Error-free and error-prone lesion bypass by human DNA polymerase kappa in vitro

Geacintov, NE, Zhang, Y, Yuan, F, Wang, M, Taylor, JS, Wang, Z, Rechkoblit, O, Wu, X

Nucleic Acids Res. 2000
11006276 Fidelity and processivity of DNA synthesis by DNA polymerase kappa, the product of the human DINB1 gene

Gerlach, VL, Feaver, WJ, Friedberg, EC, Kunkel, TA, Matsuda, T, Ohashi, E, Bebenek, K, Ohmori, H

J. Biol. Chem. 2000
12145297 Human DNA polymerase kappa bypasses and extends beyond thymine glycols during translesion synthesis in vitro, preferentially incorporating correct nucleotides

Hatahet, Z, Friedberg, EC, Gerlach, VL, Fischhaber, PL, Feaver, WJ, Wallace, SS

J. Biol. Chem. 2002
22721435 Replication of the 2,6-diamino-4-hydroxy-N(5)-(methyl)-formamidopyrimidine (MeFapy-dGuo) adduct by eukaryotic DNA polymerases

Rizzo, CJ, Christov, PP, Lloyd, RS, Wood, RD, Takata, K, Choi, JY, Guengerich, FP, Yamanaka, K

Chem. Res. Toxicol. 2012
19492058 Structure of human DNA polymerase kappa inserting dATP opposite an 8-OxoG DNA lesion

Johnson, RE, Vasquez-Del Carpio, R, Prakash, L, Aggarwal, AK, Lone, S, Silverstein, TD, Swan, MK, Choudhury, JR, Prakash, S

PLoS ONE 2009
12444249 Role of human DNA polymerase kappa as an extender in translesion synthesis

Haracska, L, Prakash, L, Prakash, S

Proc. Natl. Acad. Sci. U.S.A. 2002
20660785 Error-free replicative bypass of thymine glycol by the combined action of DNA polymerases kappa and zeta in human cells

Prakash, L, Yoon, JH, Bhatia, G, Prakash, S

Proc. Natl. Acad. Sci. U.S.A. 2010
19170759 Identification of a novel REV1-interacting motif necessary for DNA polymerase kappa function

Vaziri, C, Kamei, K, Hashimoto, H, Song, I, Ohmori, H, Ohashi, E, Hanafusa, T, Tomida, J

Genes Cells 2009
22772988 Preferred WMSA catalytic mechanism of the nucleotidyl transfer reaction in human DNA polymerase ? elucidates error-free bypass of a bulky DNA lesion

Broyde, S, Wang, L, Geacintov, NE, Zhang, Y, Wang, S, Lior-Hoffmann, L

Nucleic Acids Res. 2012
15475561 Quantitative analysis of translesion DNA synthesis across a benzo[a]pyrene-guanine adduct in mammalian cells: the role of DNA polymerase kappa

Avkin, S, Friedberg, EC, Velasco-Miguel, S, Geacintov, N, Livneh, Z, Goldsmith, M

J. Biol. Chem. 2004
11784855 Stimulation of DNA synthesis activity of human DNA polymerase kappa by PCNA

Johnson, RE, Haracska, L, Hurwitz, J, Prakash, L, Phillips, BB, Unk, I, Prakash, S

Mol. Cell. Biol. 2002
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