Reactome | POLK forms a quaternary complex with REV1 and POLZ on damaged DNA template

POLK forms a quaternary complex with REV1 and POLZ on damaged DNA template

Stable Identifier

R-HSA-5655835

Type

Reaction [binding]

Species

Homo sapiens

Compartment

nucleoplasm

ReviewStatus

5/5

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POLK forms a quaternary complex with REV1 and POLZ on damaged DNA template

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DNA polymerase kappa (POLK) forms a quaternary complex with REV1 and the DNA polymerase zeta (POLZ) heterodimer, composed of REV3L and MAD2L2 (REV7), at DNA damage sites (Ohashi et al. 2009, Wojtaszek et al. 2012a, Wojtaszek et al. 2012b, Xie et al. 2012). POLK simultaneously interacts with the C-terminus of REV1 through its RIR (REV1-interacting region) (Ohashi et al. 2009) and with monoubiquitinated PCNA (Haracska et al. 2002, Bi et al. 2006). POLK requires Mg2+ or Mn2+ for its activity. POLK is more catalytically active in the presence of Mn2+, but exhibits higher fidelity in the presence of Mg2+ (Pence et al. 2012)

Literature References

PubMed ID	Title	Journal	Year
23143872	Structural insights into the assembly of human translesion polymerase complexes Xu, M, Yang, X, Xie, W, Jiang, T	Protein Cell	2012
22859295	Structural basis of Rev1-mediated assembly of a quaternary vertebrate translesion polymerase complex consisting of Rev1, heterodimeric polymerase (Pol) ?, and Pol ? Kim, H, D'Souza, S, Minesinger, B, Lee, CJ, Zhou, P, Walker, GC, Wojtaszek, J, D'Andrea, AD	J. Biol. Chem.	2012
20952260	Bypass of N²-ethylguanine by human DNA polymerase ? Perrino, FW, Blans, P, Zink, CN, Pence, MG, Fishbein, JC	DNA Repair (Amst.)	2011
16611994	Rad18 regulates DNA polymerase kappa and is required for recovery from S-phase checkpoint-mediated arrest Vaziri, C, Tateishi, S, Barkley, LR, Yamaizumi, M, Bi, X, Ohmori, H, Slater, DM	Mol. Cell. Biol.	2006
19170759	Identification of a novel REV1-interacting motif necessary for DNA polymerase kappa function Vaziri, C, Kamei, K, Hashimoto, H, Song, I, Ohmori, H, Ohashi, E, Hanafusa, T, Tomida, J	Genes Cells	2009
22700975	Multifaceted recognition of vertebrate Rev1 by translesion polymerases ? and ? D'Souza, S, Wang, S, Zhou, P, Liu, J, Walker, GC, Wojtaszek, J, Xue, Y	J. Biol. Chem.	2012
11784855	Stimulation of DNA synthesis activity of human DNA polymerase kappa by PCNA Johnson, RE, Haracska, L, Hurwitz, J, Prakash, L, Phillips, BB, Unk, I, Prakash, S	Mol. Cell. Biol.	2002