FEN1 bound to POLB cleaves displaced DNA strand (flap)

Stable Identifier
R-HSA-5651782
Type
Reaction [transition]
Species
Homo sapiens
Compartment
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POLB-bound FEN1 (flap endonuclease) cleaves the displaced DNA strand (flap structure), which contains the damaged AP residue that could not be excised by POLB (DNA polymerase beta) (Klungland and Lindahl 1997, Prasad et al. 2001, Liu et al. 2005).

Literature References
PubMed ID Title Journal Year
11440997 DNA polymerase beta -mediated long patch base excision repair. Poly(ADP-ribose)polymerase-1 stimulates strand displacement DNA synthesis

Prasad, R, Lavrik, OI, Kim, SJ, Kedar, P, Yang, XP, Vande Berg, BJ, Wilson, SH

J. Biol. Chem. 2001
15561706 DNA polymerase beta and flap endonuclease 1 enzymatic specificities sustain DNA synthesis for long patch base excision repair

Liu, Y, Beard, WA, Shock, DD, Prasad, R, Hou, EW, Wilson, SH

J. Biol. Chem. 2005
9214649 Second pathway for completion of human DNA base excision-repair: reconstitution with purified proteins and requirement for DNase IV (FEN1).

Klungland, A, Lindahl, T

EMBO J 1997
Participants
Participant Of
Catalyst Activity
Catalyst Activity
Title
5'-flap endonuclease activity of FEN1:POLB:SSB(3'poly-dNMP-displaced 5'ddRP-FLAP)-dsDNA [nucleoplasm]
Physical Entity
Activity
Authored
Reviewed
Created