FEN1 bound to POLB cleaves displaced DNA strand (flap)

Stable Identifier
Reaction [transition]
Homo sapiens
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POLB-bound FEN1 (flap endonuclease) cleaves the displaced DNA strand (flap structure), which contains the damaged AP residue that could not be excised by POLB (DNA polymerase beta) (Klungland and Lindahl 1997, Prasad et al. 2001, Liu et al. 2005).

Literature References
PubMed ID Title Journal Year
15561706 DNA polymerase beta and flap endonuclease 1 enzymatic specificities sustain DNA synthesis for long patch base excision repair

Liu, Y, Beard, WA, Wilson, SH, Prasad, R, Hou, EW, Shock, DD

J. Biol. Chem. 2005
11440997 DNA polymerase beta -mediated long patch base excision repair. Poly(ADP-ribose)polymerase-1 stimulates strand displacement DNA synthesis

Vande Berg, BJ, Wilson, SH, Kim, SJ, Kedar, P, Yang, XP, Lavrik, OI, Prasad, R

J. Biol. Chem. 2001
9214649 Second pathway for completion of human DNA base excision-repair: reconstitution with purified proteins and requirement for DNase IV (FEN1).

Lindahl, T, Klungland, A

EMBO J 1997
Catalyst Activity

5'-flap endonuclease activity of FEN1:POLB:SSB(3'poly-dNMP-displaced 5'ddRP-FLAP)-dsDNA [nucleoplasm]

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