Reactome | mRNA decay by 5' to 3' exoribonuclease

mRNA decay by 5' to 3' exoribonuclease

Stable Identifier

R-HSA-430039

Type

Pathway

Species

Homo sapiens

Compartment

cytosol

ReviewStatus

5/5

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Degradation of mRNA from 5' to 3' occurs in three steps. First, the mRNA is bound at its 3' end by the Lsm1-7 complex. The bound Lsm1-7 may prevent nucleases from accessing the 3' end. Second, the 7-methylguanosine cap of the mRNA is hydrolyzed by the DCP1-DCP2 complex. Third, the 5' end of the decapped mRNA is attacked by the XRN1 exoribonuclease which digests the remainder of the mRNA from 5' to 3'. These processes may be physically connected by PATL1, the homolog of yeast Pat1, which stably binds the Lsm1-7 complex and interacts with the DCP1-DCP2 decapping complex and the Ccr4-NOT deadenylation complex (Ozgur et al. 2010).

Literature References

PubMed ID	Title	Journal	Year
14749774	The enzymes and control of eukaryotic mRNA turnover Parker, R, Song, H	Nat Struct Mol Biol	2004
16141059	From birth to death: the complex lives of eukaryotic mRNAs Moore, MJ	Science	2005
15475613	Messenger RNA decay in mammalian cells: the exonuclease perspective Fritz, DT, Bergman, N, Kilpatrick, WJ, Wilusz, CJ, Wilusz, J	Cell Biochem Biophys	2004
20584987	Human Pat1b connects deadenylation with mRNA decapping and controls the assembly of processing bodies Ozgur, S, Chekulaeva, M, Stoecklin, G	Mol Cell Biol	2010
19239894	The many pathways of RNA degradation Houseley, J, Tollervey, D	Cell	2009
17245413	The highways and byways of mRNA decay Garneau, NL, Wilusz, J, Wilusz, CJ	Nat Rev Mol Cell Biol	2007
11283721	The cap-to-tail guide to mRNA turnover Wilusz, CJ, Wormington, M, Peltz, SW	Nat Rev Mol Cell Biol	2001