Reactome | isocitrate + NADP+ => 2-oxoglutarate + CO2 + NADPH + H+

isocitrate + NADP+ => 2-oxoglutarate + CO2 + NADPH + H+

Stable Identifier

R-HSA-389540

Type

Reaction [transition]

Species

Homo sapiens

Compartment

cytosol

ReviewStatus

5/5

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isocitrate + NADP+ => 2-oxoglutarate + CO2 + NADPH + H+

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Cytosolic IDH1 (isocitrate dehydrogenase 1) homodimer catalyzes the reaction of isocitrate and NADP+ to form 2-oxoglutarate, CO2, and NADPH + H+. The same enzyme can also localize to peroxisomes (Geisbrecht and Gould 1999; Xu et al. 2004). IDH1 doesn't seem to be part of a hypothetical cytosolic TCA cycle because its product 2-OG would have to be reimported into the mitochondrial matrix. However, the only known transporter of 2-OG, SLC25A12, mainly exports it in exchange with oxoadipate under normal physiological conditions (Fiermonte et al., 2001).

Literature References

PubMed ID	Title	Journal	Year
10521434	The human PICD gene encodes a cytoplasmic and peroxisomal NADP(+)-dependent isocitrate dehydrogenase Geisbrecht, BV, Gould, SJ	J Biol Chem	1999
15173171	Structures of human cytosolic NADP-dependent isocitrate dehydrogenase reveal a novel self-regulatory mechanism of activity Xu, X, Zhao, J, Xu, Z, Peng, B, Huang, Q, Arnold, E, Ding, J	J Biol Chem	2004
11083877	Identification of the human mitochondrial oxodicarboxylate carrier. Bacterial expression, reconstitution, functional characterization, tissue distribution, and chromosomal location Fiermonte, G, Dolce, V, Palmieri, L, Ventura, M, Runswick, MJ, Palmieri, F, Walker, JE	J Biol Chem	2001