DOHH:Fe2+ hydroxylates Dhp-K50-EIF5A to form Hyp-K50-EIF5A

Stable Identifier
Reaction [transition]
Homo sapiens
EIF5A(Dhp) + O2 => EIF5A(Hyp)
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Cytosolic deoxyhypusine hydroxylase (DOHH) complexed with Fe2+ catalyzes the irreversible hydroxylation of peptidyl deoxyhypusine (Dhp-K50-EIF5A) to peptidyl hypusine (Hyp-K50-EIF5A) using molecular oxygen. The only known substrate for this enzyme is the modified lysine at residue 50 of the two isoforms of eIF5A (Clement et al. 2003; Kang et al. 2007; Kim et al. 2006).

Literature References
PubMed ID Title Journal Year
16533814 Deoxyhypusine hydroxylase is a Fe(II)-dependent, HEAT-repeat enzyme. Identification of amino acid residues critical for Fe(II) binding and catalysis [corrected].

Wolff, EC, Park, MH, Kim, YS, Bell, JK, McPhie, P, Kang, KR

J Biol Chem 2006
14622290 Identification and characterization of eukaryotic initiation factor 5A-2

Wolff, EC, Henderson, CA, Park, MH, Johansson, HE, Hershey, JW, Clement, PM, Smit-McBride, Z, Jenkins, ZA

Eur J Biochem 2003
17213197 Specificity of the deoxyhypusine hydroxylase-eukaryotic translation initiation factor (eIF5A) interaction: identification of amino acid residues of the enzyme required for binding of its substrate, deoxyhypusine-containing eIF5A

Wolff, EC, Park, MH, Kim, YS, Kang, KR

J Biol Chem 2007
Catalyst Activity

deoxyhypusine monooxygenase activity of DOHH:Fe++ [cytosol]

Orthologous Events
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