Reactome | DOHH:Fe2+ hydroxylates Dhp-K50-EIF5A to form Hyp-K50-EIF5A

DOHH:Fe2+ hydroxylates Dhp-K50-EIF5A to form Hyp-K50-EIF5A

Stable Identifier

R-HSA-204662

Type

Reaction [transition]

Species

Homo sapiens

Compartment

cytosol

Synonyms

EIF5A(Dhp) + O2 => EIF5A(Hyp)

ReviewStatus

5/5

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DOHH:Fe2+ hydroxylates Dhp-K50-EIF5A to form Hyp-K50-EIF5A

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Cytosolic deoxyhypusine hydroxylase (DOHH) complexed with Fe2+ catalyzes the irreversible hydroxylation of peptidyl deoxyhypusine (Dhp-K50-EIF5A) to peptidyl hypusine (Hyp-K50-EIF5A) using molecular oxygen. The only known substrate for this enzyme is the modified lysine at residue 50 of the two isoforms of eIF5A (Clement et al. 2003; Kang et al. 2007; Kim et al. 2006).

Literature References

PubMed ID	Title	Journal	Year
16533814	Deoxyhypusine hydroxylase is a Fe(II)-dependent, HEAT-repeat enzyme. Identification of amino acid residues critical for Fe(II) binding and catalysis [corrected]. Kim, YS, Kang, KR, Wolff, EC, Bell, JK, McPhie, P, Park, MH	J Biol Chem	2006
14622290	Identification and characterization of eukaryotic initiation factor 5A-2 Clement, PM, Henderson, CA, Jenkins, ZA, Smit-McBride, Z, Wolff, EC, Hershey, JW, Park, MH, Johansson, HE	Eur J Biochem	2003
17213197	Specificity of the deoxyhypusine hydroxylase-eukaryotic translation initiation factor (eIF5A) interaction: identification of amino acid residues of the enzyme required for binding of its substrate, deoxyhypusine-containing eIF5A Kang, KR, Kim, YS, Wolff, EC, Park, MH	J Biol Chem	2007