DOHH:Fe2+ hydroxylates Dhp-K50-EIF5A to form Hyp-K50-EIF5A

Stable Identifier
R-HSA-204662
Type
Reaction [transition]
Species
Homo sapiens
Compartment
Synonyms
EIF5A(Dhp) + O2 => EIF5A(Hyp)
ReviewStatus
5/5
Locations in the PathwayBrowser
General
SVG |   | PPTX  | SBGN
Click the image above or here to open this reaction in the Pathway Browser
The layout of this reaction may differ from that in the pathway view due to the constraints in pathway layout
Cytosolic deoxyhypusine hydroxylase (DOHH) complexed with Fe2+ catalyzes the irreversible hydroxylation of peptidyl deoxyhypusine (Dhp-K50-EIF5A) to peptidyl hypusine (Hyp-K50-EIF5A) using molecular oxygen. The only known substrate for this enzyme is the modified lysine at residue 50 of the two isoforms of eIF5A (Clement et al. 2003; Kang et al. 2007; Kim et al. 2006).
Literature References
PubMed ID Title Journal Year
16533814 Deoxyhypusine hydroxylase is a Fe(II)-dependent, HEAT-repeat enzyme. Identification of amino acid residues critical for Fe(II) binding and catalysis [corrected].

Wolff, EC, Park, MH, Kim, YS, Bell, JK, McPhie, P, Kang, KR

J Biol Chem 2006
14622290 Identification and characterization of eukaryotic initiation factor 5A-2

Wolff, EC, Henderson, CA, Park, MH, Johansson, HE, Hershey, JW, Clement, PM, Smit-McBride, Z, Jenkins, ZA

Eur J Biochem 2003
17213197 Specificity of the deoxyhypusine hydroxylase-eukaryotic translation initiation factor (eIF5A) interaction: identification of amino acid residues of the enzyme required for binding of its substrate, deoxyhypusine-containing eIF5A

Wolff, EC, Park, MH, Kim, YS, Kang, KR

J Biol Chem 2007
Participants
Participates
Catalyst Activity

deoxyhypusine monooxygenase activity of DOHH:Fe++ [cytosol]

Orthologous Events
Authored
Reviewed
Created
Cite Us!