The formate-tetrahydrofolate ligase activity of the trifunctional MTHFD1 enzyme catalyzes the reaction of THF polyglutamate, formate, and ATP to form 10-formylTHF polyglutamate, ADP, and orthophosphate. MTHFD1 is cytosolic and occurs as a dimer. The human enzyme has been identified and partially characterized biochemically (Hum et al. 1988); additional reaction details can be inferred from the properties of the well-studied homologous rabbit enzyme (Villar et al. 1985).
MacKenzie, RE, Rozen, R, Hum, DW, Bell, AW
Schuster, B, Villar, E, Schirch, V, Peterson, D
formate-tetrahydrofolate ligase activity of MTHFD1 dimer [cytosol]
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