NMNAT2 transfers an adenylyl group from ATP to NAMN to yield NAAD

Stable Identifier
R-HSA-197235
Type
Reaction [transition]
Species
Homo sapiens
Compartment
Synonyms
Nicotinate D-ribonucleotide + ATP => deamino-NAD+ + pyrophosphate [NMNAT2]
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NMNAT2 catalyzes the reaction of nicotinate D-ribonucleotide and ATP to form deamino-NAD+ (nicotinate adenine dinucleotide) and pyrophosphate (Sorci et al. 2007). The active form of the enzyme is a monomer in vitro; Mg2+ is required for activity (Raffaelli et al. 2002; Sorci et al. 2007). Although the predicted amino acid sequence of the enzyme lacks an obvious signal sequence or transmembrane domain (Yalowitz et al. 2004), recombinant FLAG-tagged protein expressed in HeLa cells localizes predominantly to the Golgi apparatus (Berger et al. 2005). Its localization within the Golgi apparatus is unknown and the annotation here is based on the plausible but speculative assumption that the enzyme is associated with the Gogi membrane and accessible from the cytosol. Immunostaining studies indicate that the protein is abundant in Islets of Langerhans and in several regions of the brain (Yalowitz et al. 2004).

Literature References
PubMed ID Title Journal Year
12359228 Identification of a novel human nicotinamide mononucleotide adenylyltransferase

Raffaelli, N, Sorci, L, Amici, A, Emanuelli, M, Mazzola, F, Magni, G

Biochem Biophys Res Commun 2002
14516279 Characterization of human brain nicotinamide 5'-mononucleotide adenylyltransferase-2 and expression in human pancreas

Yalowitz, JA, Xiao, S, Biju, MP, Antony, AC, Cummings, OW, Deeg, MA, Jayaram, HN

Biochem J 2004
17402747 Initial-rate kinetics of human NMN-adenylyltransferases: substrate and metal ion specificity, inhibition by products and multisubstrate analogues, and isozyme contributions to NAD+ biosynthesis

Sorci, L, Cimadamore, F, Scotti, S, Petrelli, R, Cappellacci, L, Franchetti, P, Orsomando, G, Magni, G

Biochemistry 2007
16118205 Subcellular compartmentation and differential catalytic properties of the three human nicotinamide mononucleotide adenylyltransferase isoforms

Berger, F, Lau, C, Dahlmann, M, Ziegler, M

J Biol Chem 2005
Participants
Participant Of
Catalyst Activity
Catalyst Activity
Title
nicotinate-nucleotide adenylyltransferase activity of NMNAT2:Mg2+ [Golgi membrane]
Physical Entity
Activity
Orthologous Events
Cross References
Rhea
Authored
Created