Reactome | deoxycytidine, thymidine, or deoxyuridine + ATP => dCMP, TMP, or dUMP + ADP [TK2]

deoxycytidine, thymidine, or deoxyuridine + ATP => dCMP, TMP, or dUMP + ADP [TK2]

Stable Identifier

R-HSA-109759

Type

Reaction [transition]

Species

Homo sapiens

Compartment

mitochondrial matrix

ReviewStatus

5/5

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deoxycytidine, thymidine, or deoxyuridine + ATP => dCMP, TMP, or dUMP + ADP [TK2]

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Mitochondrial thymidine kinase 2 (TK2) catalyzes the reactions of deoxycytidine, thymidine, and deoxyuridine with ATP to form the corresponding deoxynucleotide monophosphates and ADP. The enzyme has been purified from human spleen and is active as a monomer (Munch-Petersen et al. 1991). The enzyme requires divalent cations for activity (Mg++ is preferred) but the nature of the association between the metal ion and the enzyme polypeptide is unclear (Lee and Cheng 1976). The mitochondrial localization of the enzyme has been established experimentally for rats and cattle (Jansson et al. 1992); its mitochondrial localization in humans is inferred from these results and the presence of a mitochondrial localization motif at the amino terminus of the open reading frame of a cloned human cDNA that is missing from the mature catalytically active protein (Wang et al. 1999).

Literature References

PubMed ID	Title	Journal	Year
9989599	Human thymidine kinase 2: molecular cloning and characterisation of the enzyme activity with antiviral and cytostatic nucleoside substrates Wang, L, Bergman, T, Jornvall, H, Herrstrom-Sjoberg, A, Hellman, U, Munch-Petersen, B, Eriksson, S	FEBS Lett	1999
1063125	Human deoxythymidine kinase. I. Purification and general properties of the cytoplasmic and mitochondrial isozymes derived from blast cells of acute myelocytic leukemia Cheng, YC, Lee, LS	J Biol Chem	1976
2026611	Diverging substrate specificity of pure human thymidine kinases 1 and 2 against antiviral dideoxynucleosides Cloos, L, Tyrsted, G, Munch-Petersen, B, Eriksson, S	J Biol Chem	1991
1597187	Mammalian thymidine kinase 2. Direct photoaffinity labeling with [32P]dTTP of the enzyme from spleen, liver, heart and brain Bohman, C, Munch-Petersen, B, Eriksson, S, Jansson, O	Eur J Biochem	1992