ATP-dependent release of CDT1 from the OCCM complex in budding yeast

Stable Identifier
R-SCE-9749381
Type
Reaction [transition]
Species
Saccharomyces cerevisiae
Compartment
nucleoplasm
ReviewStatus
5/5
General
SVG | 
PNG
Low
Medium
High
 | PPTX  | SBGN
ATP-dependent release of CDT1 from the OCCM complex in budding yeast
In the budding yeast, once the initial complex of ORC(1-6), CDC6, CDT1 and MCM2-7 (OCCM) is formed, CDT1 is released from DNA in an ATP-dependent manner. The ATPase activity of CDC6 is necessary for CDT1 release, but ATPase activities of other ATPases in the complex (e.g. ORC1) may contribute to CDT1 release. ORC6 is necessary for the retention of MCM2-7 after ATP hydrolysis (Fernández-Cid et al. 2013).
Literature References
PubMed ID Title Journal Year
23603117 An ORC/Cdc6/MCM2-7 complex is formed in a multistep reaction to serve as a platform for MCM double-hexamer assembly

Fernández-Cid, A, Speck, C, Samel, S, Gardenal, E, Winkler, C, Riera, A, Herrera, MC, Evrin, C, Uhle, S, Tognetti, S

Mol Cell 2013
Participants
Input
Output
Catalyst Activity

ATP hydrolysis activity of yORC(1-6):yCDC6:origin of replication:yCDT1:yMCM2-7 [nucleoplasm]

Physical Entity
yORC(1-6):yCDC6:origin of replication:yCDT1:yMCM2-7 [nucleoplasm] (Saccharomyces cerevisiae)
Activity
ATP hydrolysis activity (GO:0016887)
Orthologous Events
ATP-dependent release of CDT1 from the OCCM complex (Homo sapiens)
Authored
Kusic-Tisma, J  (2021-10-27)
Created
Orlic-Milacic, M  (2021-08-02)
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