Rpl30:Met-tRNAi:mRNA:Secisbp2:Sec-tRNA(Sec):Eefsec:GTP is hydrolysed to Rpl30:Met-tRNAi:mRNA:Secisbp2:Sec and Eefsec:GDP by Eefsec

Stable Identifier
R-RNO-5333610
Type
Reaction [transition]
Species
Rattus norvegicus
Compartment
cytosol
General
SVG | 
PNG
Low
Medium
High
 | PPTX  | SBGN
Rpl30:Met-tRNAi:mRNA:Secisbp2:Sec-tRNA(Sec):Eefsec:GTP is hydrolysed to Rpl30:Met-tRNAi:mRNA:Secisbp2:Sec and Eefsec:GDP by Eefsec

The complex consisting of selenocysteinyl (Sec) transfer RNA (tRNA) for Sec (Sec-tRNASec), selenocysteine-specific elongation factor (Eefsec), selenocysteine insertion sequence-binding protein 2 (Secisbp2) aka Sbp2 and GTP interacts with the 80S ribosomal protein L30 (Rpl30). This triggers the hydrolysis of GTP (to GDP) by Eefsec and delivery of Sec-tRNASec to the ribosomal A site (Chavatte et al. 2005). Before the Sec residue is inserted into the nascent polypeptide chain, Eefsec:GDP is released.

Literature References
PubMed ID Title Journal Year
15821744 Ribosomal protein L30 is a component of the UGA-selenocysteine recoding machinery in eukaryotes

Chavatte, L, Driscoll, DM, Brown, BA

Nat. Struct. Mol. Biol. 2005
Participants
Input
Output
Catalyst Activity

GTPase activity of Rpl30:Met-tRNAi:mRNA:Secisbp2:Sec-tRNA(Sec):Eefsec:GTP [cytosol]

Physical Entity
Rpl30:Met-tRNAi:mRNA:Secisbp2:Sec-tRNA(Sec):Eefsec:GTP [cytosol] (Rattus norvegicus)
Activity
GTPase activity (GO:0003924)
Orthologous Events
80S:Met-tRNAi:mRNA:SECISBP2:Sec-tRNA(Sec):EEFSEC:GTP is hydrolysed to 80S:Met-tRNAi:mRNA:SECISBP2:Sec and EEFSEC:GDP by EEFSEC (Homo sapiens)
Authored
Williams, MG  (2014-05-06)
Reviewed
Rush, MG  (2015-08-30)
Created
Williams, MG  (2014-02-19)
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