ANKZF1 cleaves the peptidyl-tRNA in VCP hexamer:LTN1:NEMF:60S ribosome subunit:peptidyl-tRNA with K48polyUb-nascent peptide

Stable Identifier
R-HSA-9948427
Type
Reaction [transition]
Species
Homo sapiens
Compartment
cytosol
ReviewStatus
5/5
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ANKZF1 cleaves the peptidyl-tRNA in VCP hexamer:LTN1:NEMF:60S ribosome subunit:peptidyl-tRNA with K48polyUb-nascent peptide
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ANKZF1 cleaves the C-terminal 3 nucleotides, CCA, of the tRNA in the peptidyl-tRNA bound to the 60S ribosomal subunit, yielding a free tRNA and the nascent peptide covalently bound to the CCA sequence (Verma et al. 2018, Yip et al. 2019, and inferred from the yeast homolog, VMS1, in Verma et al. 2018, Yip et al. 2019). In yeast, Arb1 (mammalian ABCF2) occupies the E-site of the collided ribosome, extending a domain towards the peptidyl-tRNA that may help position it for release by Vms1/ANKZF1 (Su et al. 2019). ANKZF1 interacts strongly with VCP (Hänzelmann and Schindelin 2011).
Literature References
PubMed ID Title Journal Year
31011209 Mechanism for recycling tRNAs on stalled ribosomes

Yip, MCJ, Keszei, AFA, Feng, Q, Chu, V, McKenna, MJ, Shao, S

Nat Struct Mol Biol 2019
30244831 Release of Ubiquitinated and Non-ubiquitinated Nascent Chains from Stalled Mammalian Ribosomal Complexes by ANKZF1 and Ptrh1

Kuroha, K, Zinoviev, A, Hellen, CUT, Pestova, TV

Mol Cell 2018
29632312 Vms1 and ANKZF1 peptidyl-tRNA hydrolases release nascent chains from stalled ribosomes

Verma, R, Reichermeier, KM, Burroughs, AM, Oania, RS, Reitsma, JM, Aravind, L, Deshaies, RJ

Nature 2018
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Catalyst Activity

RNA endonuclease activity of ANKZF1 [cytosol]

Physical Entity
ANKZF1 [cytosol] (Homo sapiens)
Activity
RNA endonuclease activity (GO:0004521)
Authored
May, B  (2025-05-20)
Reviewed
Bennett, EJ  (2025-08-09)
Ford, PW  (2025-08-09)
Jafarnejad, SM  (2025-11-07)
McGirr, T  (2025-11-07)
Created
May, B  (2025-05-20)
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