Reactome | LTN1 and TCF25 bind NEMF:60S ribosomal subunit:peptidyl-tRNA with nascent peptide-Ala

LTN1 and TCF25 bind NEMF:60S ribosomal subunit:peptidyl-tRNA with nascent peptide-Ala

Stable Identifier

R-HSA-9948318

Type

Reaction [binding]

Species

Homo sapiens

Compartment

cytosol

ReviewStatus

5/5

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LTN1 and TCF25 bind NEMF:60S ribosomal subunit:peptidyl-tRNA with nascent peptide-Ala

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The N-terminal region of Listerin (LTN1), a ubiquitin E3 ligase, contacts NEMF bound to the peptidyl-tRNA and the 60S ribosomal subunit. The C-terminal region of LTN1 binds the 60S ribosomal subunit near the exit tunnel (Shao et al. 2015, inferred from yeast homologs in Lyumkis et al. 2014). NEMF is required for LTN1 binding to the 60S ribosome (Shao et al. 2015). TCF25 (the human homolog of RQC1 in yeast) interacts with LTN1 (inferred from yeast homologs in Defenouillère et al. 2013). After binding the 60S ribosomal subunit, LTN1 then conjugates ubiquitin to lysine residues of the nascent peptide that protrudes from the exit tunnel (reviewed in Mishra et al. 2021)

Literature References

PubMed ID	Title	Journal	Year
40169231	The ribosome-associated quality control factor TCF25 imposes K48 specificity on Listerin-mediated ubiquitination of nascent chains by binding and specifically orienting the acceptor ubiquitin Abaeva, IS, Bulakhov, AG, Hellen, CUT, Pestova, TV	Genes Dev	2025
25578875	Structure and assembly pathway of the ribosome quality control complex Shao, S, Brown, A, Santhanam, B, Hegde, RS	Mol Cell	2015