TBX21 and RUNX3 bind the IFNG gene

Stable Identifier
R-HSA-9942723
Type
Reaction [binding]
Species
Homo sapiens
Compartment
nucleoplasm
ReviewStatus
5/5
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TBX21 and RUNX3 bind the IFNG gene
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TBX21 (Beima et al. 2006, Jenner et al. 2009, Henderson et al. 2021, and inferred from mouse homologs in Djuretic et al. 2007, Zhu et al. 2012) and RUNX3 (inferred from mouse homologs in Djuretic et al. 2007) bind to the upstream promoter of the interferon gamma (IFNG) gene. In mouse, Tbx21 also binds a conserved noncoding sequence region (CNS) about 22 kb upstream of the transcription start site of the Ifng gene (CNS-22), which is essential for expression of the Ifng gene in Th1 cells (Hatton et al. 2006). In mouse chromatin immunoprecipitation assays, Tbx21 is observed to bind CNS-6 6kb upstream of the Ifng transcription start site (Zhu et al 2012). A comparison between human and mouse IFNG genes indicates that TBX21 binds 4 conserved upstream sites and 3 conserved downstream sites (Kanhere et al. 2012). TBX21 and RUNX3 then enhance transcription of IFNG (Jenner et al. 2009, and inferred from mouse homologs in Szabo et al. 2000, Mullen et al. 2001, Hatton et al. 2006, Djuretic et al. 2007, Zhu et al. 2012).
Literature References
PubMed ID Title Journal Year
34531288 The Th1 cell regulatory circuitry is largely conserved between human and mouse

Henderson, S, Pullabhatla, V, Hertweck, A, de Rinaldis, E, Herrero, J, Lord, GM, Jenner, RG

Life Sci Alliance 2021
16473879 T-bet binding to newly identified target gene promoters is cell type-independent but results in variable context-dependent functional effects

Beima, KM, Miazgowicz, MM, Lewis, MD, Yan, PS, Huang, TH, Weinmann, AS

J Biol Chem 2006
23232398 T-bet and GATA3 orchestrate Th1 and Th2 differentiation through lineage-specific targeting of distal regulatory elements

Kanhere, A, Hertweck, A, Bhatia, U, Gökmen, MR, Perucha, E, Jackson, I, Lord, GM, Jenner, RG

Nat Commun 2012
19805038 The transcription factors T-bet and GATA-3 control alternative pathways of T-cell differentiation through a shared set of target genes

Jenner, RG, Townsend, MJ, Jackson, I, Sun, K, Bouwman, RD, Young, RA, Glimcher, LH, Lord, GM

Proc Natl Acad Sci U S A 2009
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Tbx21 (T-bet) and Runx3 bind the Ifng gene (Mus musculus)
Authored
May, B  (2025-03-17)
Reviewed
Barbutti, I  (2025-08-04)
Lord, GM  (2025-08-05)
Created
May, B  (2025-03-19)
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