Reactome | SMARCE1 and SMARCB1 bind SMARCC1 dimer:SMARCD1

SMARCE1 and SMARCB1 bind SMARCC1 dimer:SMARCD1

Stable Identifier

R-HSA-9932447

Type

Reaction [binding]

Species

Homo sapiens

Compartment

nucleoplasm

ReviewStatus

5/5

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SMARCE1 and SMARCB1 bind SMARCC1 dimer:SMARCD1

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After formation of the cBAF core trimer consisting of SMARCC1 and SMARCD1, SMARCE1 and SMARCB1 are recruited (Mashtalir et al, 2018). Crosslinking and knockout studies indicate that the SMARCE1 subunit of the cBAF complex is directly recruited through interaction with the SMARCC1 dimer (Mashtalir et al, 2018; Mashtalir et al, 2020). Together, the SMARCC homodimer, SMARCD1, SMARCE1 and SMARCB1 form the cBAF core module that lies adjacent to the nucleosome (Nakayama et al, 2017; Mashtalir et al, 2018; Mashtalir et al, 2020; He et al, 2020). Knockdown of SMARCE1 destabilizes cBAF complex assembly, particularly affecting the recruitment of ARID subunits, while knockdown of SMARCB1 shows minimal effect on cBAF complex assembly but more pronounced effects on pBAF assembly. SMARCE1 and SMARCB1 are dispensable for the assembly of the ncBAF complex (Mashtalir et al, 2018). SMARCB1 has a positive C-terminal alpha-helix that mediates contacts with acidic patches on the nucleosome (He et al, 2020; Mashtalir et al, 2020). Mutations in SMARCB1, common in pediatric rhabdoid tumors, affect the targeting of the SWI/SNF complex to enhancers such that recruitment at "super-enhancers" is maintained at the expense of regular, non-super-enhancer regions (Wang et al, 2017; Nakayama et al, 2017).

Literature References

PubMed ID	Title	Journal	Year
28945250	SMARCB1 is required for widespread BAF complex-mediated activation of enhancers and bivalent promoters Nakayama, RT, Pulice, JL, Valencia, AM, McBride, MJ, McKenzie, ZM, Gillespie, MA, Ku, WL, Teng, M, Cui, K, Williams, RT, Cassel, SH, Qing, H, Widmer, CJ, Demetri, GD, Irizarry, RA, Zhao, K, Ranish, JA, Kadoch, C	Nat Genet	2017
27941797	SMARCB1-mediated SWI/SNF complex function is essential for enhancer regulation Wang, X, Lee, RS, Alver, BH, Haswell, JR, Wang, S, Mieczkowski, J, Drier, Y, Gillespie, SM, Archer, TC, Wu, JN, Tzvetkov, EP, Troisi, EC, Pomeroy, SL, Biegel, JA, Tolstorukov, MY, Bernstein, BE, Park, PJ, Roberts, CW	Nat Genet	2017
33053319	A Structural Model of the Endogenous Human BAF Complex Informs Disease Mechanisms Mashtalir, N, Suzuki, H, Farrell, DP, Sankar, A, Luo, J, Filipovski, M, D'Avino, AR, St Pierre, R, Valencia, AM, Onikubo, T, Roeder, RG, Han, Y, He, Y, Ranish, JA, Dimaio, F, Walz, T, Kadoch, C	Cell	2020
32001526	Structure of nucleosome-bound human BAF complex He, S, Wu, Z, Tian, Y, Yu, Z, Yu, J, Wang, X, Li, J, Liu, B, Xu, Y	Science	2020
30343899	Modular Organization and Assembly of SWI/SNF Family Chromatin Remodeling Complexes Mashtalir, N, D'Avino, AR, Michel, BC, Luo, J, Pan, J, Otto, JE, Zullow, HJ, McKenzie, ZM, Kubiak, RL, St Pierre, R, Valencia, AM, Poynter, SJ, Cassel, SH, Ranish, JA, Kadoch, C	Cell	2018