PKG phosphorylates NS5

Stable Identifier
R-HSA-9920025
Type
Reaction [transition]
Species
Homo sapiens
Related Species
Dengue virus type 2
Compartment
cytosol
ReviewStatus
5/5
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PKG phosphorylates NS5
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NS5 from DENV-2 (strain U87411) is phosphorylated at Thr-449 (Thr-2940 of the polyprotein) in transfected human HEK293T cells; this specific threonine residue in bacterially-expressed recombinant NS5 is also a target for in vitro phosphorylation by activated bovine protein kinase G (Bhattacharya et al., 2009). We inferred the reaction catalyzed by human PKG from the bovine PKG activity.
Literature References
PubMed ID Title Journal Year
7642575 Association between NS3 and NS5 proteins of dengue virus type 2 in the putative RNA replicase is linked to differential phosphorylation of NS5

Kapoor, M, Zhang, L, Ramachandra, M, Kusukawa, J, Ebner, KE, Padmanabhan, R

J Biol Chem 1995
19587048 Protein kinase G phosphorylates mosquito-borne flavivirus NS5

Bhattacharya, D, Mayuri, N, Best, SM, Perera, R, Kuhn, RJ, Striker, R

J Virol 2009
Participants
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Output
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as an event of
Catalyst Activity

cGMP-dependent protein kinase activity of Activated cGMP-dependent protein kinase (PKGs) [cytosol]

Physical Entity
Activated cGMP-dependent protein kinase (PKGs) [cytosol] (Homo sapiens)
Activity
cGMP-dependent protein kinase activity (GO:0004692)
Inferred From
PKG phosphorylates NS5 (Bos taurus)
Disease
Name Identifier Synonyms
dengue disease DOID:12205 breakbone fever, Dengue Fever, classic dengue
Cross References
RHEA
Mondo
Authored
Stephan, R  (2024-08-19)
Reviewed
Mansur, DS  (2025-09-18)
Roque Ascenção, F  (2025-10-29)
Created
Stephan, R  (2024-08-29)
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