PKG phosphorylates NS5

Stable Identifier
R-BTA-9954423
Type
Reaction [transition]
Species
Bos taurus
Related Species
Dengue virus type 2
Compartment
cytosol
ReviewStatus
5/5
General
SVG | 
PNG
Low
Medium
High
 | PPTX  | SBGN
PKG phosphorylates NS5
NS5 from DENV-2 (strain U87411) is phosphorylated at Thr-449 (Thr-2940 of the polyprotein) in infected human HEK293T cells; this specific threonine residue in bacterially-expressed recombinant NS5 is also a target for in vitro phosphorylation by activated bovine protein kinase G (Bhattacharya et al., 2009).
Literature References
PubMed ID Title Journal Year
7642575 Association between NS3 and NS5 proteins of dengue virus type 2 in the putative RNA replicase is linked to differential phosphorylation of NS5

Kapoor, M, Zhang, L, Ramachandra, M, Kusukawa, J, Ebner, KE, Padmanabhan, R

J Biol Chem 1995
19587048 Protein kinase G phosphorylates mosquito-borne flavivirus NS5

Bhattacharya, D, Mayuri, N, Best, SM, Perera, R, Kuhn, RJ, Striker, R

J Virol 2009
Participants
Input
Output
Catalyst Activity

cGMP-dependent protein kinase activity of cGMP-dependent protein kinase 1, alpha isozyme [cytosol]

Physical Entity
cGMP-dependent protein kinase 1, alpha isozyme [cytosol] (Bos taurus)
Activity
cGMP-dependent protein kinase activity (GO:0004692)
Orthologous Events
PKG phosphorylates NS5 (Homo sapiens)
Disease
Name Identifier Synonyms
dengue disease DOID:12205 breakbone fever, Dengue Fever, classic dengue
Cross References
RHEA
Mondo
Authored
Stephan, R  (2025-06-04)
Reviewed
Roque Ascenção, F  (2025-10-29)
Mansur, DS  (2025-10-29)
Created
Stephan, R  (2025-06-07)
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