Reactome | POLRMT polymerizes RNA primer for mitochondrial L strand replication

POLRMT polymerizes RNA primer for mitochondrial L strand replication

Stable Identifier

R-HSA-9914409

Type

Reaction [omitted]

Species

Homo sapiens

Compartment

mitochondrial matrix

ReviewStatus

5/5

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POLRMT polymerizes RNA primer for mitochondrial L strand replication

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POLRMT initiates transcription at a stem-loop structure in OriL and polymerizes a primer RNA of approximately 25 nt (Wanrooij et al. 2008, Fusté et al. 2010, Sarfallah et al. 2021, inferred in vivo from mice in Wanrooij et al. 2008). The transcription factor TFAM is not required for transcription initiation at OriL (Fusté et al. 2010). Slippage of the transcript at 6 deoxythymidine residues results in an extra 2 adenosine residues being incorporated in the RNA primer and this slippage is required for stability of the initiation complex (Sarfallah et al. 2021)

Literature References

PubMed ID	Title	Journal	Year
18685103	Human mitochondrial RNA polymerase primes lagging-strand DNA synthesis in vitro Wanrooij, S, Fusté, JM, Farge, G, Shi, Y, Gustafsson, CM, Falkenberg, M	Proc Natl Acad Sci U S A	2008
20129056	Mitochondrial RNA polymerase is needed for activation of the origin of light-strand DNA replication Fusté, JM, Wanrooij, S, Jemt, E, Granycome, CE, Cluett, TJ, Shi, Y, Atanassova, N, Holt, IJ, Gustafsson, CM, Falkenberg, M	Mol Cell	2010
34423452	Mechanism of transcription initiation and primer generation at the mitochondrial replication origin OriL Sarfallah, A, Zamudio-Ochoa, A, Anikin, M, Temiakov, D	EMBO J	2021

Participants

Input

Output

Participates

as an event of

Strand-asynchronous mitochondrial DNA replication (Homo sapiens)

Catalyst Activity

DNA-directed RNA polymerase activity of POLRMT:POLG:PEO1:OriL:Mitochondrial H strand replisome:H strand RNA primer:nascent H strand DNA [mitochondrial matrix]

Physical Entity

POLRMT:POLG:PEO1:OriL:Mitochondrial H strand replisome:H strand RNA primer:nascent H strand DNA [mitochondrial matrix] (Homo sapiens)

Activity

DNA-directed RNA polymerase activity (GO:0003899)

Orthologous Events

POLRMT polymerizes RNA primer for mitochondrial L strand replication (Bos taurus)

POLRMT polymerizes RNA primer for mitochondrial L strand replication (Caenorhabditis elegans)

POLRMT polymerizes RNA primer for mitochondrial L strand replication (Canis familiaris)

POLRMT polymerizes RNA primer for mitochondrial L strand replication (Danio rerio)

POLRMT polymerizes RNA primer for mitochondrial L strand replication (Drosophila melanogaster)

POLRMT polymerizes RNA primer for mitochondrial L strand replication (Gallus gallus)

POLRMT polymerizes RNA primer for mitochondrial L strand replication (Mus musculus)