Reactome | Binding of newly synthesized MHC class I heavy chain (HC) with calnexin

Binding of newly synthesized MHC class I heavy chain (HC) with calnexin

Stable Identifier

R-HSA-983145

Type

Reaction [binding]

Species

Homo sapiens

Compartment

integral component of lumenal side of endoplasmic reticulum membrane, endoplasmic reticulum lumen

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Binding of newly synthesized MHC class I heavy chain (HC) with calnexin

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The newly synthesized MHC class I heavy chain (HC) translocates into the endoplasmic reticulum (ER) and binds rapidly to calnexin (CNX), a transmembrane calcium-dependent lectin with chaperone activity. CNX recognizes and binds to an N-linked monoglycosylated Glc1Man9GlcNAc2 carbohydrate group found attached to the conserved Asn-86 in the HC. Interaction of HC with CNX promotes the formation of intrachain disulfide bonds. Another candidate ER chaperon protein is immunoglobulin binding protein (BiP), found to associate with HC in the absence of CNX. These chaperones can cooperate in protein folding and prevention of aggregation.

Literature References

PubMed ID	Title	Journal	Year
7642652	Calnexin influences folding of human class I histocompatibility proteins but not their assembly with beta 2-microglobulin Tector, M, Salter, RD	J Biol Chem	1995
11160214	A role for calnexin in the assembly of the MHC class I loading complex in the endoplasmic reticulum Diedrich, G, Bangia, N, Pan, M, Cresswell, P	J Immunol	2001
9605141	Calreticulin and calnexin interact with different protein and glycan determinants during the assembly of MHC class I Harris, MR, Yu, YY, Kindle, CS, Hansen, TH, Solheim, JC	J Immunol	1998
7876241	The molecular chaperone calnexin binds Glc1Man9GlcNAc2 oligosaccharide as an initial step in recognizing unfolded glycoproteins Ware, FE, Vassilakos, A, Peterson, PA, Jackson, MR, Lehrman, MA, Williams, DB	J Biol Chem	1995
10777614	Functional relationship between calreticulin, calnexin, and the endoplasmic reticulum luminal domain of calnexin Danilczyk, UG, Cohen-Doyle, MF, Williams, DB	J Biol Chem	2000
11514579	Relationship between calnexin and BiP in suppressing aggregation and promoting refolding of protein and glycoprotein substrates Stronge, VS, Saito, Y, Ihara, Y, Williams, DB	J Biol Chem	2001